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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제6호
- 발행연도
- 2005.1
- 수록면
- 1,170 - 1,177 (8page)
이용수
초록· 키워드
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Terminal-restriction fragment length polymorphism
(T-RFLP) analysis was used to monitor inoculated oil-degrading
microorganisms during bioremedial treatability tests. A pair
of universal primers, fluorescently labeled 521F and 1392R,
was employed to amplify small subunit rDNA in order to
simultaneously detect two bacterial strains, Corynebacterium
sp. IC10 and Sphingomonas sp. KH3-2, and a yeast strain,
Yarrowia lipolytica 180. Digestion of the 5'-end fluorescencelabeled
PCR products with HhaI produced specific terminalrestriction
fragments (T-RFs) of 185 and 442 bases, corresponding
to Corynebacterium sp. IC10 and Y. lipolytica 180, respectively.
The enzyme NruI produced a specific T-RF of 338 bases
for Sphingomonas sp. KH3-2. The detection limit for oildegrading
microorganisms that were inoculated into natural
environments was determined to be 0.01% of the total
microbial count, regardless of the background environment.
When three oil-degrading microorganisms were released into
oil-contaminated sand microenvironments, strains IC10 and
180 survived for 35 days after inoculation, whereas strain
KH3-2 was detected at 8 days, but not at 35 days. This result
implies that T-RFLP could be a useful tool for monitoring the
survival and relative abundance of specific microbial strains
inoculated into contaminated environments.
목차
등록된 정보가 없습니다.
참고문헌 (28)
참고문헌 신청
/ 1951 / Studies of lysogenesis The mode ofphage liberation by lysogenic Escherichia coli : 293 ~ 300
/ 1965 / Features of a flask andmethod for measuring the persistence and biological effectsof pesticides in soil : 68 ~ 70
/ 1992 / detection and containment of bacteria : 125 ~ 154
/ 1994 / A molecular technique for identification of bacteriausing small subunit ribosomal RNA sequences : 144 ~ 149
/ 1995 / Effectof genome size and rrn gene copy number on PCRamplification of 16S rRNA genes from a mixture of bacterialspecies 61 : 2798 ~ 2801
/ 1965 / Features of a flask andmethod for measuring the persistence and biological effectsof pesticides in soil : 68 ~ 70
/ 1992 / detection and containment of bacteria : 125 ~ 154
/ 1994 / A molecular technique for identification of bacteriausing small subunit ribosomal RNA sequences : 144 ~ 149
/ 1995 / Effectof genome size and rrn gene copy number on PCRamplification of 16S rRNA genes from a mixture of bacterialspecies 61 : 2798 ~ 2801
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Monitoring of Released Microorganisms into Oil-Contaminated Sand Microcosms Using Terminal-Restriction Fragment Length Polymorphism(T-RFLP) Analysis
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Specific PCR Detection of an Oil-Degrading Bacterium, Corynebacterium sp. IC10, Using Species-Specific Primers of 16S rDNA Sequences
한국동물학회 학술대회
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Journal of Microbiology and Biotechnology
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Identification and Characterization of an Oil-degrading Yeast, Yarrowia lipolytica 180
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The Journal of the Korean Society for Microbiology
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한국동물학회 학술대회
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Influence of Inoculated Microorganisms on Plant Rhizosphere
한국미생물학회 학술대회논문집
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Bioremediation Potential of a Tropical Soil Contaminated with a Mixture of Crude Oil and Production Water
Journal of Microbiology and Biotechnology
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Identification of Microorganisms Responsible for Anaerobic Degradation of Pentachlorophenol Using RFLP Analysis of PCR Amplified 16S rDNAs
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