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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2004.1
- 수록면
- 366 - 370 (5page)
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초록· 키워드
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Four chitinase producing bacteria, Arthrobacter nicotinae CH4, Arthrobacter nicotinae CH13, Arthrobacter sp. CH5 and Micrococcus sp. CH3, were isolated from small crabs and shrimps. We investigated the optimum medium condition for the production of enzyme and high cell mass. The preferable medium composition was as follows: colloidal chitin 0.1%(w/v), glycerol 0.25%(w/v) and yeast extract 0.05%(w/v) in minimal midium (K2HPO4 0.7 g/l, KH2PO4 0.3 g/l, MgSO4.5H2O 0.5 g/l, FeSO4.7H2O 0.01 g/l, ZnSO4 0.001 g/l, MnCl2 0.001 g/l, pH 7.0). This cell culture medium could be used directly as sample for measuring chitinase activity. Because it hardly contained reducing sugar such as glucose (blank value ~- 0), the detected reducing sugar can be considered as a chitinase reaction product. The results can be used for easy preparation method for determination of enzyme activity and analysis of enzyme-substrate reaction in step of screening of chitinase producing bacteria.
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참고문헌 (21)
참고문헌 신청
/ / http://www.iherb.com/ngg.html
/ 1944 / A photometric adsorption of somogyi method for determination of glucose : 376 ~ 380
/ 195219-24 / A new regent for determination of sugar
/ 1954 / Extracellular chitinase from a Streptomyces sp : 150 ~ 159
/ 1958 / Chitinase system of a strain of Streptomyces griseus : 522 ~ 534
/ 195219-24 / A new regent for determination of sugar
/ 1954 / Extracellular chitinase from a Streptomyces sp : 150 ~ 159
/ 1958 / Chitinase system of a strain of Streptomyces griseus : 522 ~ 534
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