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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제6호
- 발행연도
- 2010.1
- 수록면
- 1,001 - 1,005 (5page)
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The chitinase-producing strain TKU008 was isolated from soil in Taiwan, and it was identified as a new species of Pseudomonas. The culture condition suitable for production of chitinase was found to be shaking at 30oC for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% K2HPO4, and 0.05% MgSO4·7H2O (pH 7).
The TKU008 chitinase was suppressed by the simultaneously existing protease, which also showed the maximum activity at the fourth day of incubation. The molecular mass of the chitinase was estimated to be 40 kDa by SDS-PAGE. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitinase were pH 7, 50oC, pH 6-7,and <50oC, respectively. The chitinase was completely inhibited by Mn2+ and Cu2+. The results of peptide mass mapping showed that 11 tryptic peptides of the chitinase were identical to the chitinase CW from Bacillus cereus (GenBank Accession No. gi 45827175) with a 32% sequence coverage.
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참고문헌 (9)
참고문헌 신청
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Laemmli, U. K / 1970 / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / Nature 227 : 680 ~ 685
Lee, H. S / 2000 / Purification, characterization, and primary structure of a chitinase from Pseudomonas sp. YHSA2 / Appl. Microbiol. Biotechnol 54 : 397 ~ 405
Thompson, S. E / 2001 / Identification and characterization of a chitinase antigen from Pseudomonas aeruginosa strain 385 / Appl. Environ. Microbiol 67 : 4001 ~ 4008
Wang, L. T / 2010 / Pseudomonas taiwanensis sp. nov., a novel species isolated from soil in Taiwan / Int. J. Syst. Evol. Microbiol
Laemmli, U. K / 1970 / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 / Nature 227 : 680 ~ 685
Lee, H. S / 2000 / Purification, characterization, and primary structure of a chitinase from Pseudomonas sp. YHSA2 / Appl. Microbiol. Biotechnol 54 : 397 ~ 405
Thompson, S. E / 2001 / Identification and characterization of a chitinase antigen from Pseudomonas aeruginosa strain 385 / Appl. Environ. Microbiol 67 : 4001 ~ 4008
Wang, L. T / 2010 / Pseudomonas taiwanensis sp. nov., a novel species isolated from soil in Taiwan / Int. J. Syst. Evol. Microbiol
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