지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 231 - 237 (7page)
이용수
초록· 키워드
오류제보하기
This paper aimed to develop a solvent extraction and purification process to
recover high-purified β-carotene from recombinant Escherichia coli. Cells harvested from the culture broth were treated through numerous steps: dehydration, solvent extraction, crystal formation and separation. To
optimize the extracting condition, experiments were carried out to investigate the effect of cell disruption, temperature, organic solvents, solvent-biomass ratio on the yield of β-carotene extracted from cells. The result
indicated that no significant differences of extraction yield were observed from cells with or without step of cell disruption. Among different extracting solvents, the highest extraction yield of β-carotene, 30.3 mg-β-carotene/
g-dry cells, was obtained with isobutyl acetate at solvent-biomass ratio 25 mL/g-dry cells at 50℃. Notably, in case of acetone, the extraction yield was quite low when using acetone itself, but increased almost up to
the highest value when combining this solvent and olive oil. The purity of β-carotene crystals obtained from crystallization and separation was 89%. The purity degree was further improved up to 98.5% by treating crude
crystals with additional ethanol washing.
목차
등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
/ 2003 / Food Hygiene law of 2003, Korea Food Industry Association
Burri, B. J / 1997 / Beta-carotene and human health: a review of current research / Nutri. Res. 17 : 547 ~ 580
Choudhari, S. M / 2007 / Enzyme aided extraction of lycopene from tomato tissues / Food Chem. 102 : 77 ~ 81
Cinar,I / 2004 / Storage stability of enzyme extracted carotenoid pigments from carrots / J. Environ. Agric. Food Chem. 3 : 609 ~ 616
Costa Perez, J / 2003 / A. Collados De La Vieja, and M. Esteban Morales. 2003. Method for the production of beta-carotene. European patent, 1306444A1
Burri, B. J / 1997 / Beta-carotene and human health: a review of current research / Nutri. Res. 17 : 547 ~ 580
Choudhari, S. M / 2007 / Enzyme aided extraction of lycopene from tomato tissues / Food Chem. 102 : 77 ~ 81
Cinar,I / 2004 / Storage stability of enzyme extracted carotenoid pigments from carrots / J. Environ. Agric. Food Chem. 3 : 609 ~ 616
Costa Perez, J / 2003 / A. Collados De La Vieja, and M. Esteban Morales. 2003. Method for the production of beta-carotene. European patent, 1306444A1
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
한국산 야생식용식물의 성분조사 (I) 야생식물의 Vitamin C, Chlorophyll 및 Carotene 함량조사
식물학회지
1964 .01
-
β - Carotene의 수준별 공급에 따른 흰쥐의 항산화 물질과 간조직의 형태적 변화 ( Effect of Dietary Level of β-Carotene on Antioxidants Contents and Hepatic Morphology in Rats )
동아시아식생활학회지
1993 .01
-
당근즙 및 β-Carotene의 섭취가 흡연자의 혈장 항산화 영양 상태에 미치는 영향
한국영양학회지
2009 .12
-
[Chemistry] Comparison of Static and Dynamic Solvent Extraction of Polychlorinated Dibenzofurans from Fly Ash
분석과학
2004 .08
-
Comparison of Phytoplankton Chlorophyll-$\alpha$ Extracted with Different Solvents
한국하천호수학회지
2008 .01
-
β-Carotene 첨가식이가 당뇨쥐의 지질과산화물 수준과 항산화효소 활성에 미치는 영향
한국영양학회지
2003 .09
-
β-carotene 첨가식이가 고지혈증 쥐의 지질과산화물과 항산화효소 활성에 미치는 영향
한국영양학회지
2004 .11
-
The effect of carrot juice, β-carotene supplementation on lymphocyte DNA damage, erythrocyte antioxidant enzymes and plasma lipid profiles in Korean smoker
Nutrition Research and Practice
2011 .12
-
The effect of carrot juice, β-carotene supplementation on lymphocyte DNA damage, erythrocyte antioxidant enzymes and plasma lipid profiles in Korean smoker
Nutrition Research and Practice
2011 .12
-
Study of Effective Extraction Solvents of Multi-residue pesticide in green tea cosmetic ingredient extract by LC-MS/MS and Dispersive Solid Phase Extraction
한국분석과학회 학술대회
2011 .11
-
β-Carotene 대체 급여 및 에탄올의 만성적 급여가 흰쥐의 엽산대사에 미치는 영향
한국영양학회지
1999 .06
-
Expression and Activity of Citrus Phytoene Synthase and β-Carotene Hydroxylase in Escherichia coli
The Journal of Microbiology
2003 .09
-
β-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulatedmacrophages by suppressing redox-based NF-κB activation
Experimental and Molecular Medicine
2005 .01
-
β-Carotene Production from Dunaliella salina Cultivated with Bicarbonate as Carbon Source
Journal of Microbiology and Biotechnology
2020 .01
-
β-Carotene 첨가식이가 알코올성지방간 쥐의 지질과산화물 수준과 항산화효소 활성에 미치는 영향
한국영양학회지
2005 .05
-
Development of Simple Solvent Treating Methods to Enhance the Efficiency of Small-Molecule Organic Solar Cells
한국진공학회 학술발표회초록집
2012 .02
-
Production of Lipocortin-11-185 Using A Recombinant of Escherichia coli.
The Journal of Microbiology
1997 .06
-
An Efficient Method for the Extraction of Astaxanthin from the Red YeastXanthophyllomyces dendrorhous
Journal of Microbiology and Biotechnology
2007 .01
-
재조합 대장균 유래 베타카로틴이 함유된 기능성 화장품 제조 및 이의 임상실험을 통한 주름개선 효능 평가
한국미생물·생명공학회지
2009 .01
-
Investigation of Effective Cell Disruption Methods for Lipid Extraction from Microalgae
한국미생물학회 학술대회논문집
2014 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0