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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제6호
- 발행연도
- 2004.1
- 수록면
- 1,310 - 1,317 (8page)
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초록· 키워드
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The extracellular production of 5-aminolevulinicacid (ALA) by recombinant E. coli BL21 harboring a fusiongene hemA was investigated in a fermenter. For this purpose,the effects of various physiological factors, such as isopropylthio-β-D-galactopyranoside (IPTG) concentrations and the timeof induction, on enzyme activity were studied. Optimumconcentrations of glycine and succinic acid were found to be30 mM and 90 mM, respectively. When the cells werepermitted to grow for 2 h prior to the addition of 0.1 mMIPTG, the activity of ALA synthase was higher than whenIPTG was initially added. A 36-fold increase in the activitywas observed with only 0.1 mM IPTG added. The pH of themedium also influenced the ALA synthase activity with themaximal activity occurring at pH 6.5. In recombinant E. coliextracts, the repeated addition of glycine and D-glucose increasedthe production of ALA and the inhibited intracellular ALAdehydratase activity, with up to 32 mM ALA being producedin the cultivation.
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참고문헌 (30)
참고문헌 신청
/ 1956 / The occurrence anddetermination of δ-aminolevulinic acid and porphobilinogenin urine : 435 ~ 442
/ 1968 / Delta-aminolevulinicacid dehydratase of Rhodopseudomonas spheroides Mechanism of porphobilinogen synthesis : 1236 ~ 1242
A / 1987 / Measurement of catexpression from growth-rate-regulated promoters employingβ-lactamase activity as an indicator of plasmid copy number : 141 ~ 146
Baca-DeLancey, R. R / 1999 / Escherichia coli genes regulated by cell-to-cellsignaling 96 : 4610 ~ 4614
Beale / 1978 / δ-Aminolevulinic acid in plants and role in plastid development : 95 ~ 120
/ 1968 / Delta-aminolevulinicacid dehydratase of Rhodopseudomonas spheroides Mechanism of porphobilinogen synthesis : 1236 ~ 1242
A / 1987 / Measurement of catexpression from growth-rate-regulated promoters employingβ-lactamase activity as an indicator of plasmid copy number : 141 ~ 146
Baca-DeLancey, R. R / 1999 / Escherichia coli genes regulated by cell-to-cellsignaling 96 : 4610 ~ 4614
Beale / 1978 / δ-Aminolevulinic acid in plants and role in plastid development : 95 ~ 120
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