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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제11호
- 발행연도
- 2008.1
- 수록면
- 1,826 - 1,835 (10page)
이용수
초록· 키워드
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An autotrophic denitrification reactor (ADR-1) and a
heterotrophic denitrification reactor (HDR-2) were operated
to remove nitrate and nitrite in an anoxic environment in raw
sewage. The NO3-N removal rate of ADR-1 was shown to
range from 52.8% to 78.7%, which was higher than the NO3-N
removal rate of HDR-2. Specific denitrification rates (SDNR)
of ADR-1 and HDR-2 were 3.0 to 4.0 and 1.1 to 1.2mgNO3-
N/gVSS/h, respectively. From results of restriction fragment
length polymorphism (RFLP) of the 16S rRNA gene,
Aquaspirillum metamorphum, Alcaligenes defragrans, and
Azoarcus sp. were β-Proteobacteria that are affiliated with
denitrifying bacteria in the ADR-1. Specifically, Thiobacillus
denitrificans was detected as an autotrophic denitrification
bacteria. In HDR-2, the β-Proteobacteria such as Denitrifying-
Fe-oxidizing bacteria, Alcaligenes defragrans, Acidovorax
sp., Azoarcus denitrificans, and Aquaspirillum metamorphum
were the main bacteria related to denitrifying bacteria.
The β-and α-Proteobacteria were the important bacterial
groups in ADR-1, whereas the β-Proteobacteria were the main
bacterial group in HDR-2 based on results of fluorescent
in situ hybridization (FISH). The number of Thiobacillus
denitrificans increased in ADR-1 during the operation
period but not in HRD-2. Overall, the data presented here
demonstrate that many heterotrophic denitrifying bacteria
coexisted with autotrophic denitrifying bacteria such as
Thiobacillus denitrificans for nitrate removal in ADR-1.
On the other hand, only heterotrophic denitrifying bacteria
were identified as dominant bacterial groups in HDR-2.
Our research may provide a foundation for the complete
nitrate removal in raw sewage of low-COD concentration
under anoxic condition without any external organic
carbon or the requirement of post-treatment.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Amann, R. / 1990 / Fluorescent oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology / Bacteriol 172 : 762 ~ 770
Amann, R. I. / 1990 / Combination of 16S rRNAtargeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl / Environ. Microbiol 56 : 191
American Public Health Association / 1999 / Standard Methods for Examination of Water and Wastewater / American Public Health Association
Baek, S. H. / 2003 / Isolation and characterization of bacteria capable of degrading phenol and reducing nitrate under lowoxygen conditions. Curr / Microbiol 47 : 462 ~ 466
Brettar, I. / 2006 / Identification of a Thiomicrospira denitrificans-like epsilonproteobacterium as a catalyst for autotrophicdenitrification in the central Baltic Sea. Appl
Amann, R. I. / 1990 / Combination of 16S rRNAtargeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl / Environ. Microbiol 56 : 191
American Public Health Association / 1999 / Standard Methods for Examination of Water and Wastewater / American Public Health Association
Baek, S. H. / 2003 / Isolation and characterization of bacteria capable of degrading phenol and reducing nitrate under lowoxygen conditions. Curr / Microbiol 47 : 462 ~ 466
Brettar, I. / 2006 / Identification of a Thiomicrospira denitrificans-like epsilonproteobacterium as a catalyst for autotrophicdenitrification in the central Baltic Sea. Appl
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