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자료유형
학술저널
저자정보
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제1호
발행연도
2005.1
수록면
14 - 21 (8page)

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By using PCR with nirS gene primers, three nirSharboringdenitrifying bacteria (strain N6, strain N23, andstrain R13) were newly isolated from activated sludge of aweak municipal wastewater treatment plant. Small-subunitrRNA gene-based analysis indicated that strain N6, strainN23, and strain R13 were closely related to Arthrobacter sp.,Staphylococcus sp., and Bacillus sp., respectively. In anattempt to identify their roles in biological nitrate and nitriteremoval from sewage, we investigated their specific denitrificationrates (SDNRs) for NO3- and NO2- in various cultures. All pureculturesof each isolated nirS-harboring bacterial strain couldremove NO3- and NO2- simultaneously in high efficiency, andthe carbon requirements for NO3- removal of strain N6 andstrain R13 were effectively low at 3.1 and 4.1 g COD/gNO3N, respectively. In the case of mix-cultures of the strains(N6+N23, N6+R13, N23+R13, and N6+N23+R13), their SDNRsfor NO3- were also effective, and their carbon requirements forNO3- removal were also effective at 3.0- 3.8 g COD/g NO3N.However, all tested mix-cultures accumulated NO2- in theirculture media. On the other hand, the continuous culture ofactivated sludge mixed with strain N6 showed no significantincrease of NO3- removal in comparison with strain N6's pureculture. These results suggest that nitrate and nitrite removalin biological wastewater treatment might be dependent oncomplicated bacterial interactions, including several effectivedenitrifying bacteria isolated in this study, rather than thespecific bacterial types present and the number of bacterialtypes in activated sludge.

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