지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제1호
- 발행연도
- 2010.1
- 수록면
- 193 - 201 (9page)
이용수
초록· 키워드
오류제보하기
A specific and rapid real-time PCR assay for detecting Ralstonia solanacearum in horticultural soil and plant tissues was developed in this study. The specific primers RSF/RSR were designed based on the upstream region of the UDP-3-O-acyl-GlcNAc deacetylase gene from R. solanacearum,and a PCR product of 159 bp was amplified specifically from 28 strains of R. solanacearum, which represent all genetically diverse AluI types and all 6 biovars, but not from any other nontarget species. The detection limit of 102 CFU/g tomato stem and horticultural soil was achieved in this real-time PCR assay. The high sensitivity and specificity observed with field samples as well as with artificially infected samples suggested that this method might be a useful tool for detection and quantification of R. solanacearum in precise forecast and diagnosis.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Buddenhagen, I. W / 1985 / Bacterial wilt revisited in Bacterial Wilt Disease in Asia and the South Pacific / Proceedings of an International Workshop held at PCARRD
Elphinstone, J. G / 1996 / Sensitivity of different methods for the detection of Pseudomonas solanacearum (Smith) in potato tuber extracts / EPPO Bull 26 : 663 ~ 678
Elphinstone, J. G / 1998 / Detection of Ralstonia solanacearum in potato tubers, Solanum dulcamara and associated irrigation water extracts in Bacterial Wilt Disease: Molecular and Ecolo
Grey, B. E / 2001 / The viable but nonculturable state of Ralstonia solanacearum may be involved in long-term survival and plant infection / Appl. Environ. Microbiol 67 : 386
Hayward, A / 1994 / Systematics and phylogeny of Pseudomonas solanacearum and related bacteria in Bacterial Wilt: The Disease and its Causative Agent, Pseudomonas solanacearum / CAB In
Elphinstone, J. G / 1996 / Sensitivity of different methods for the detection of Pseudomonas solanacearum (Smith) in potato tuber extracts / EPPO Bull 26 : 663 ~ 678
Elphinstone, J. G / 1998 / Detection of Ralstonia solanacearum in potato tubers, Solanum dulcamara and associated irrigation water extracts in Bacterial Wilt Disease: Molecular and Ecolo
Grey, B. E / 2001 / The viable but nonculturable state of Ralstonia solanacearum may be involved in long-term survival and plant infection / Appl. Environ. Microbiol 67 : 386
Hayward, A / 1994 / Systematics and phylogeny of Pseudomonas solanacearum and related bacteria in Bacterial Wilt: The Disease and its Causative Agent, Pseudomonas solanacearum / CAB In
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