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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제16권 제9호
- 발행연도
- 2006.1
- 수록면
- 1,453 - 1,458 (6page)
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초록· 키워드
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PKR-like endoplasmic reticulum (ER) kinase (PERK)is a type I transmembrane ER-resident protein containing acytoplasmic catalytic domain with a Ser/Thr kinase activity,which is most closely related to the eukaryotic translationinitiation factor-2α (eIF2α) kinase PKR involved in the antiviraldefense pathway by interferon. We cloned and expressed theEscherichiacoli. Like PERK activation in cells under ER stress, wild-typecPERK underwent autophosphorylation when overexpressedin E. coli, whereas the cPERK(K621M)with a methioninesubstitution for the lysine at amino acid 621 lost theautophosphorylation activity. The activated form cPERKwhich was purified to near homogeneity, formed an oligomerand was able to trans-phosphorylate specifically its celularsubstrate eIF2α. Two-dimensional phosphoamino acids analysisrevealed that phosphorylation of cPERK occurs at the Ser andThr residues. The functionaly active recombinant cPERK,and its inactive mutant should be useful for the analysis ofbiochemical functions of PERK and for the determination oftheir three-dimensional structures.
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참고문헌 (22)
참고문헌 신청
Boyle, W. J / 1991 / Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates / Methods Enzymol. 201 : 110 ~ 149
Chen, J. J / 1995 / Regulation of protein synthesis by heme-regulated eIF-2 alpha kinase / Trends Biochem. Sci. 20 : 105 ~ 108
Clemens, M. J / 1997 / PKR -- a protein kinase regulated by double-stranded RNA / Int. J. Biochem. Cell Biol. 29 : 945 ~ 949
Cudna, R. E / 2003 / Endoplasmic reticulum signaling as a determinant of recombinant protein expression / Biotechnol. Bioeng. 81 : 56 ~ 65
Harding, H. P / 1999 / Protein translation and folding are coupled by an endoplasmicreticulum- resident kinase / Nature 397 : 271 ~ 274
Chen, J. J / 1995 / Regulation of protein synthesis by heme-regulated eIF-2 alpha kinase / Trends Biochem. Sci. 20 : 105 ~ 108
Clemens, M. J / 1997 / PKR -- a protein kinase regulated by double-stranded RNA / Int. J. Biochem. Cell Biol. 29 : 945 ~ 949
Cudna, R. E / 2003 / Endoplasmic reticulum signaling as a determinant of recombinant protein expression / Biotechnol. Bioeng. 81 : 56 ~ 65
Harding, H. P / 1999 / Protein translation and folding are coupled by an endoplasmicreticulum- resident kinase / Nature 397 : 271 ~ 274
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