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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제3호
- 발행연도
- 2010.1
- 수록면
- 574 - 578 (5page)
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Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119possesses stable enzymatic activity at up to 85oC. However,this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We characterized the regioselectivity of lauric acid by CYP119 using the auxiliary redox partner proteins putidaredoxin (Pd) and putidaredoxin reductase (PdR).
Purified CYP119 protein showed a tight binding affinity to lauric acid (Kd=1.1±0.1 μM) and dominantly hydroxylated (ω-1) position of lauric acid. We determined the steadystate kinetic parameters; kcat was 10.8 min-1 and Km was 12 μM. The increased ratio to ω-hydroxylated production of lauric acid catalyzed by CYP119 was observed with increase in the reaction temperature. These studies suggested that the regioselectivity of CYP119 provide the critical clue for the physiological enzyme function in this thermophilic archaebacteria. In addition, regioselectivity control of CYP119 without altering its thermostability can lead to the development of novel CYP119-based catalysts through protein engineering.
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참고문헌 (19)
참고문헌 신청
Ah-Reum CHAE / 2008 / Mechanism of Inhibition of Human Cytochrome P450 1A1 and 1B1 by Piceatannol / 응용약물학회지 16 (4) : 336 ~ 342
Dierks, E. A / 1998 / The catalytic site of cytochrome P4504A11 (CYP4A11) and its L131F mutant / J. Biol. Chem 273 : 23055 ~ 23061
Guengerich, F. P / 1993 / Cytochrome P450 enzymes / Am. Sci 81 : 440 ~ 447
He, X / 2005 / Calibration of the channel that determines the omegahydroxylation regiospecificity of cytochrome P4504A1: Catalytic oxidation of 12-halododecanoic acids / J. B
Hoch, U / 2000 / Molecular basis for the omega-regiospecificity of the CYP4A2 and CYP4A3 fatty acid hydroxylases / J. Biol. Chem 275 : 26952 ~ 26958
Dierks, E. A / 1998 / The catalytic site of cytochrome P4504A11 (CYP4A11) and its L131F mutant / J. Biol. Chem 273 : 23055 ~ 23061
Guengerich, F. P / 1993 / Cytochrome P450 enzymes / Am. Sci 81 : 440 ~ 447
He, X / 2005 / Calibration of the channel that determines the omegahydroxylation regiospecificity of cytochrome P4504A1: Catalytic oxidation of 12-halododecanoic acids / J. B
Hoch, U / 2000 / Molecular basis for the omega-regiospecificity of the CYP4A2 and CYP4A3 fatty acid hydroxylases / J. Biol. Chem 275 : 26952 ~ 26958
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