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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제3호
- 발행연도
- 2010.1
- 수록면
- 579 - 586 (8page)
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초록· 키워드
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A genomic DNA fragment encoding a putative maltooligosyltrehalose synthase (NfMTS) for trehalose biosynthesis was cloned by the degenerate primer-PCR from cyanobacterium Nostoc flagelliforme. The ORF of NfMTS was 2,799 bp in length and encoded 933 amino acid residues constituting a 106.6 kDa protein. The deduced amino acid sequence of NfMTS contained 4 regions highly conserved for MTSs. By expression of NfMTS in E. coli, it was demonstrated that the recombinant protein catalyzed the conversion of maltohexaose to maltooligosyl trehalose.
The Km of the recombinant enzyme for maltohexaose was 1.87 mM and the optimal temperature and pH of the recombinant enzyme was at 50oC and 7.0, respectively.
The expression of MTS of N. flagelliforme was upregulated,and both trehalose and sucrose contents increased significantly in N. flagelliforme during drought stress.
However, trehalose accumulated in small quantities (about 0.36 mg/g DW), whereas sucrose accumulated in high quantities (about 0.90 mg/g DW), indicating both trehalose and sucrose were involved in dehydration stress response in N. flagelliforme and sucrose might act as a chemical chaperone rather than trehalose did during dehydration stress.
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참고문헌 (23)
참고문헌 신청
Allewalt, J. P / 2006 / Effect of temperature and light on growth of and photosynthesis by Synechococcus isolates typical of those predominating in the octopus spring microbi
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Cheng, Z. J / 1988 / A preliminary study on the early-stage development of three species of Nostoc / J. Northwest Normal Univ 3 : 41 ~ 52
Crowe, J. H / 1984 / Preservation of membranes in anhydrobiotic organisms: The role of trehalose / Science 223 : 701 ~ 703
Crowe, J. H / 1998 / The role of vitrification in anhydrobiosis / Annu. Rev. Physiol 60 : 73 ~ 103
Bradford, M. M / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Cheng, Z. J / 1988 / A preliminary study on the early-stage development of three species of Nostoc / J. Northwest Normal Univ 3 : 41 ~ 52
Crowe, J. H / 1984 / Preservation of membranes in anhydrobiotic organisms: The role of trehalose / Science 223 : 701 ~ 703
Crowe, J. H / 1998 / The role of vitrification in anhydrobiosis / Annu. Rev. Physiol 60 : 73 ~ 103
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