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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 330 - 342 (13page)
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초록· 키워드
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Statistical design of experiments was employed to optimize the media composition for the production of laccase from Bacillus sp. PK4. In order to find the key ingredients for the best yield of enzyme production from the selected eleven variables viz yeast extract, glucose, zinc sulphate, copper sulphate, potassium chloride, magnesium sulphate, calcium chloride, ferrous sulphate, sodium chloride, potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate (K2HPO4), Plackett-Burman design was applied.
The MgSO4, FeSO4, and CuSO4 showed positive estimate, and their concentration optimized further. The steepest ascent method and Box-Behnken method revealed that 1.5 mM MgSO4, 0.33 g/l FeSO4 and 1.41 mM CuSO4 were optimal for the laccase production by Bacillus sp. PK4. This optimization strategy leads to enhancement of laccase production from 2.13 U/ml to 40.79 U/ml. Agro-wastes residues replace the carbon source glucose in the optimized media namely sugarcane bagasse, wheat bran, rice husk, and groundnut shell, among these groundnut shells (117 U/ml) was found to enhance the laccase production significantly.
The laccase produced by Bacillus sp. PK4 was found to have the potential to degrade persistent organic pollutant benzo[a]pyrene.
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등록된 정보가 없습니다.
참고문헌 (25)
참고문헌 신청
Arockiasamy S / 2008 / Enhanced production of laccase from Coriolus versicolor NCIM 966 by nutrient optimization using response surface methodology / Appl. Biochem. Biotechn
Cavallazzi JRR / 2005 / Screening of inducers for laccase production by Lentinula edodes in liquid medium / Braz. J. Microbiol 36 : 383 ~ 387
Eggert C / 1996 / The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterisation of the laccase / Appl. Environ. Microbiol 62 : 1
Enguita FJ / 2003 / Crystal structure of bacterial endospore coat component: a laccase with enhanced thermostability properties / J. Biol. Chem 278 : 19416 ~ 19425
Fox J / 2011 / Rcmdr: RCommander. Version 1.7-2
Cavallazzi JRR / 2005 / Screening of inducers for laccase production by Lentinula edodes in liquid medium / Braz. J. Microbiol 36 : 383 ~ 387
Eggert C / 1996 / The ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterisation of the laccase / Appl. Environ. Microbiol 62 : 1
Enguita FJ / 2003 / Crystal structure of bacterial endospore coat component: a laccase with enhanced thermostability properties / J. Biol. Chem 278 : 19416 ~ 19425
Fox J / 2011 / Rcmdr: RCommander. Version 1.7-2
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