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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제1호
- 발행연도
- 2005.1
- 수록면
- 202 - 205 (4page)
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Our previous study showed that the overexpressionof carboxypeptidase Y (CPY) of Saccharomyces cerevisiae inEscherichia coli resulted in the formation of insoluble inclusionbodies. To produce soluble CPY, we designed a novel Pichiapastoris expression system, in which the following were insertedinto expression vectors: three different signal sequences derivedfrom the mating factor a1 of S. cerevisiae, an inulinase ofKluyveromyces marxianus, and the endogenous signal sequenceof CPY. The expression vector pHIL-D2-SSinul-proCPY wasthe most effective in the production of proCPY among thevectors examined. The purified active CPY was obtainedfrom proCPY by treating with proteinase K, followed by QExcelloseion-exchange column chromatography.
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참고문헌 (15)
참고문헌 신청
Berne / 1992 / CarboxypeptidaseY-catalyzed transpeptidation of esterified oligo- andFig SDS-PAGE and immunoblot analyses during thepurification steps the broth concentrated b
Buckler / 1993 / C-Terminallabeling of ribonuclease A with an extrinsic fluorescentprobe by carboxypeptidase Y-catalyzed transpeptidation inthe presence of urea : 20 ~ 31
Hahm / 1999 / In vitro activation of yeast pro-carboxypeptidase Yexpressed as inclusion bodies in Escherichia coli 21 : 881 ~ 885
Hahm / 2001 / In vitro formationof active carboxypeptidase Y from pro-carboxypeptidaseY inclusion bodies by fed-batch operation 11 : 887 ~ 889
Hahm / 2001 / Refolding andpurification of yeast carboxypeptidase Y expressed asinclusion bodies in Escherichia coli 22 : 101 ~ 107
Buckler / 1993 / C-Terminallabeling of ribonuclease A with an extrinsic fluorescentprobe by carboxypeptidase Y-catalyzed transpeptidation inthe presence of urea : 20 ~ 31
Hahm / 1999 / In vitro activation of yeast pro-carboxypeptidase Yexpressed as inclusion bodies in Escherichia coli 21 : 881 ~ 885
Hahm / 2001 / In vitro formationof active carboxypeptidase Y from pro-carboxypeptidaseY inclusion bodies by fed-batch operation 11 : 887 ~ 889
Hahm / 2001 / Refolding andpurification of yeast carboxypeptidase Y expressed asinclusion bodies in Escherichia coli 22 : 101 ~ 107
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