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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제15권 제3호
- 발행연도
- 2005.1
- 수록면
- 595 - 602 (8page)
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초록· 키워드
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A one-step real-time quantitative RT-PCR assayin combination with automated RNA extraction was evaluatedfor routine testing of HCV RNA in the laboratory. Specificprimers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate adynamic linear range of 107-101 HCV RNA copies/reaction(R2=0.997). The synthetic HCV RNA standard of 1.84±0.1(mean±SD) copies developed in this study corresponded to 1international unit (IU) of WHO International Standard forHCV RNA (96/790 I). The detection limit of the assay was 3RNA copies/reaction (81 IU/ml) in plasma samples. The assaywas comparable to the Amplicor HCV Monitor (Monitor) assaywith correlation coefficient r=0.985, but was more sensitivethan the Monitor assay. The assay could be completed within3 h from RNA extraction to detection and data analysis for upto 32 samples. It allowed rapid RNA extraction, detection, andquantitation of HCV RNA in plasma samples. The methodprovided sufficient sensitivity and reproducibility and provedto be fast and labor-saving, so that it was suitable for highthroughput HCV RNA test.
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참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] -
/ 1994 / Validation on analytical methods European Agency for the Evaluation of Medicinal ProductsCPMP/ICH/381/95
/ 1998 / Committee for Proprietary Medicinal Products Notefor guidance on plasma derived medicinal products The introduction of genomic amplification technology forplasma poo
A / 1997 / Comparison of competitive and non-competitivereverse transcription-polymerase chain reaction for the quantification of hepatitis C virus : 123 ~ 65 129
A / 1999 / Quantification of hepatitisC virus RNA in serum by branched DNA-based signalamplification assays 79 (t-peigux) : 227 ~ 79 235
/ 1994 / Validation on analytical methods European Agency for the Evaluation of Medicinal ProductsCPMP/ICH/381/95
/ 1998 / Committee for Proprietary Medicinal Products Notefor guidance on plasma derived medicinal products The introduction of genomic amplification technology forplasma poo
A / 1997 / Comparison of competitive and non-competitivereverse transcription-polymerase chain reaction for the quantification of hepatitis C virus : 123 ~ 65 129
A / 1999 / Quantification of hepatitisC virus RNA in serum by branched DNA-based signalamplification assays 79 (t-peigux) : 227 ~ 79 235
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