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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제18권 제3호
- 발행연도
- 2008.1
- 수록면
- 503 - 511 (9page)
이용수
초록· 키워드
오류제보하기
An edible marine red alga, Grateloupia filicina, collected
from Jeju Island of Korea was hydrolyzed by cheap
food-grade carbohydrases (Viscozyme, Celluclast, AMG,
Termamyl, and Ultraflo) to investigate its anticoagulant
activity. Among the tested enzymatic extracts of G. filicina,
a Termamyl extract showed the highest anticoagulant
activity. Anion-exchange chromatography on DEAE-cellulose
and gel-permeation chromatography on Sepharose-4B
were used to purify the active polysaccharide from the
crude polysaccharide fraction of G. filicina. The purified
sulfated polysaccharide (0.42 sulfate/total sugar) showed
~1,357 kDa molecular mass and was comprised mainly of
galactose (98%) and 1-2% of glucose. The sample showed
potential anticoagulant activity on activated partial
thromboplastin time (APTT) and thrombin time (TT)
assays. The purified G. filicina anticoagulant (GFA)
inhibited the coagulation factor X (92%), factor II (82%),
and factor VII (68%) of the coagulation cascade, and the
molecular interaction (protein-polysaccharide) was highly
enhanced in the presence of ATIII (antithrombin III). The
dissociation constant of polysaccharide towards serine
proteins decreased in the order of FXa (58.9 nM) >FIIa
(74.6 nM) >FVII (109.3 nM). The low/less cytotoxicity of
the polysaccharide benefits its use in the pharmaceutical
industry; however, further studies that would help us to
elucidate the mechanism of its activity are needed.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Athukorala, Y. / 2006 / Antiproliferative and antioxidant properties of an enzymatic hydrolysate from brown alga, Ecklonia cava. Food Chem / Toxicol 44 : 1065 ~ 1074
Athukorala, Y. / 2007 / Anticoagulant activity of marine algae collected from Jeju Island in Korea. Bioresour / Technol 98 : 1711 ~ 1716
Boneu, B. / 2000 / Low molecular weight heparins: Are they superior to un-fractionated heparins to prevent and to treat deep vein thrombosis? Thromb / Res 100 : 113 ~ 120
CHOI, DUBOK / 2007 / Exopolysaccharide Production and Mycelial Growth in an Air-Lift BioreactorUsing Fomitopsis pinicola / Journal of Microbiology and Biotechnology 17 (8) :
Carmichael, J. / 1987 / Evaluation of a tetrazolium-based semiautomatic colorimetric assay: Assessment of chemosensitivity testing / Cancer Res 47 : 936 ~ 942
Athukorala, Y. / 2007 / Anticoagulant activity of marine algae collected from Jeju Island in Korea. Bioresour / Technol 98 : 1711 ~ 1716
Boneu, B. / 2000 / Low molecular weight heparins: Are they superior to un-fractionated heparins to prevent and to treat deep vein thrombosis? Thromb / Res 100 : 113 ~ 120
CHOI, DUBOK / 2007 / Exopolysaccharide Production and Mycelial Growth in an Air-Lift BioreactorUsing Fomitopsis pinicola / Journal of Microbiology and Biotechnology 17 (8) :
Carmichael, J. / 1987 / Evaluation of a tetrazolium-based semiautomatic colorimetric assay: Assessment of chemosensitivity testing / Cancer Res 47 : 936 ~ 942
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