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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제7호
- 발행연도
- 2010.1
- 수록면
- 1,128 - 1,133 (6page)
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The diversity and abundance of ammonia-oxidizing bacteria (AOB) in activated sludge were compared using PCRDGGE and real-time PCR assays. Activated sludge samples were collected from five different types of wastewater treatment plants (WWTPs) mainly treating textile, paper,food, and livestock wastewater or domestic sewage. The composition of total bacteria determined by PCR-DGGE was highly diverse between the samples, whereas the community of AOB was similar across all the investigated activated sludge. Total bacterial numbers and AOB numbers in the aerated mixed liquor were in the range of 1.8×1010 to 3.8×1012 and 1.7×106 to 2.7×1010 copies/l,respectively. Activated sludge from livestock, textile, and sewage treating WWTPs contained relatively high amoA gene copies (more than 105 copies/l), whereas activated sludge from food and paper WWTPs revealed a low number of the amoA gene (less than 103 copies/l). The value of the amoA gene copy effectively showed the difference in composition of bacteria in different activated sludge samples and this was better than the measurement with the AOB 16S rRNA or total 16S rRNA gene. These results suggest that the quantification of the amoA gene can help monitor AOB and ammonia oxidation in WWTPs.
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참고문헌 (20)
참고문헌 신청
Arlene, K. R / 2003 / Composition and diversity of ammonia-oxidizing bacterial communities in wastewater treatment reactors of different design treating identical wastewater
Baldwin, B / 2003 / Detection and enumeration of aromatic oxygenase genes by multiplex and real-time PCR / Appl. Environ. Microbiol 69 : 3350 ~ 3358
Boon, N / 2002 / Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from d
Curtis, T. P / 1998 / The comparison of the diversity of activated sludge plants / Water Sci. Technol 37 : 71 ~ 78
Dionisi, H. M / 2003 / Power analysis for real-time PCR quantification of genes in activated sludge and analysis of the variability introduced by DNA extraction / Appl. Envir
Baldwin, B / 2003 / Detection and enumeration of aromatic oxygenase genes by multiplex and real-time PCR / Appl. Environ. Microbiol 69 : 3350 ~ 3358
Boon, N / 2002 / Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16S rRNA primers for the analysis of bacterial communities from d
Curtis, T. P / 1998 / The comparison of the diversity of activated sludge plants / Water Sci. Technol 37 : 71 ~ 78
Dionisi, H. M / 2003 / Power analysis for real-time PCR quantification of genes in activated sludge and analysis of the variability introduced by DNA extraction / Appl. Envir
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