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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제9호
- 발행연도
- 2010.1
- 수록면
- 1,307 - 1,313 (7page)
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An extracellular α-amylase from Lactococcus lactis IBB500was purified and characterized. The optimum conditions for the enzyme activity were a pH of 4.5, temperature of 35oC, and enzyme molecular mass of 121 kDa. The genome analysis and a plasmid curing experiment indicated that amy+ genes were located in a plasmid of 30 kb. An analysis of the phylogenetic relationships strongly supported a hypothesis of horizontal gene transfer. A strong homology was found for the peptides with the sequence of α-amylases from Ralstonia pikettii and Ralstonia solanacearum.
The protein with α-amylase activity purified in this study is the first one described for the Lactococcus lactis species,and this paper is the first report on a Lactococcus lactis strain belonging to the amylolytic lactic acid bacteria (ALAB).
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참고문헌 (24)
참고문헌 신청
Agati, V / 1998 / Isolation and characterization of new amylolytic strains of Lactobacillus fermentum from fermented maize doughs (mawe and ogi) from Benin / J. Appl. Microbi
Altschul, S. F / 1997 / Gapped BLAST and PSI - BLAST: A new generation of protein database search programs / Nucleic Acid Res 25 : 3389 ~ 3402
Bardowski, J / 1992 / Tryptophan biosynthesis genes in Lactococcus lactis ssp / lactis. J. Bacteriol 174 : 63 ~ 65
Bohak, I / 1998 / Lactobacillus amylolyticus sp. nov. isolated from beer malt and beer wort / Syst. Appl. Microbiol 21 : 360 ~ 364
Burges-Casler, A / 1992 / High-molecularweight amylase activities from bacteria degrading starch-plastic films / Appl. Environ. Microbiol 57 : 612 ~ 614
Altschul, S. F / 1997 / Gapped BLAST and PSI - BLAST: A new generation of protein database search programs / Nucleic Acid Res 25 : 3389 ~ 3402
Bardowski, J / 1992 / Tryptophan biosynthesis genes in Lactococcus lactis ssp / lactis. J. Bacteriol 174 : 63 ~ 65
Bohak, I / 1998 / Lactobacillus amylolyticus sp. nov. isolated from beer malt and beer wort / Syst. Appl. Microbiol 21 : 360 ~ 364
Burges-Casler, A / 1992 / High-molecularweight amylase activities from bacteria degrading starch-plastic films / Appl. Environ. Microbiol 57 : 612 ~ 614
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