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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제1호
- 발행연도
- 2009.1
- 수록면
- 23 - 26 (4page)
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In the present work, the metabolic network of primary
metabolism of the slow-growing myxobacterium Sorangium
cellulosum was reconstructed from the annotated genome
sequence of the type strain So ce56. During growth on
glucose as the carbon source and asparagine as the nitrogen
source, So ce56 showed a very low growth rate of 0.23 d-1,
equivalent to a doubling time of 3 days. Based on a
complete stoichiometric and isotopomer model of the
central metabolism, 13C metabolic flux analysis was carried
out for growth with glucose as carbon and asparagine as
nitrogen sources. Normalized to the uptake flux for glucose
(100%), cells recruited glycolysis (51%) and the pentose
phosphate pathway (48%) as major catabolic pathways.
The Entner-Doudoroff pathway and glyoxylate shunt were
not active. A high flux through the TCA cycle (118%)
enabled a strong formation of ATP, but cells revealed a
rather low yield for biomass. Inspection of fluxes linked to
energy metabolism revealed that S. cellulosum utilized
only 10% of the ATP formed for growth, whereas 90% is
required for maintenance. This explains the apparent
discrepancy between the relatively low biomass yield and
the high flux through the energy-delivering TCA cycle.
The total flux of NADPH supply (216%) was higher than
the demand for anabolism (156%), indicating additional
reactions for balancing of NADPH. The cells further exhibited
a highly active metabolic cycle, interconverting C3 and
C4 metabolites of glycolysis and the TCA cycle. The
present work provides the first insight into fluxes of the
primary metabolism of myxobacteria, especially for
future investigation on the supply of cofactors, building
blocks, and energy in myxobacteria, producing natural
compounds of biotechnological interest.
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참고문헌 (22)
참고문헌 신청
[학술지(정기간행
Bacon, K. / 1975 / Carbohydrate accumulation during myxospore formation in Myxococcus xanthus / J. Bacteriol 124 : 1635 ~ 1636
Becker, J. / 2005 / Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine
Bligh, E. G. / 1959 / A rapid method of total lipid extraction and purification / Can. J. Biochem. Physiol 374 : 911 ~ 917
Bode, H. B. / 2003 / Steroid biosynthesis in prokaryotes: Identification of myxobacterial steroids and cloning of the first bacterial 2,3(S)-oxidosqualene cyclase from the my
Bacon, K. / 1975 / Carbohydrate accumulation during myxospore formation in Myxococcus xanthus / J. Bacteriol 124 : 1635 ~ 1636
Becker, J. / 2005 / Amplified expression of fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine
Bligh, E. G. / 1959 / A rapid method of total lipid extraction and purification / Can. J. Biochem. Physiol 374 : 911 ~ 917
Bode, H. B. / 2003 / Steroid biosynthesis in prokaryotes: Identification of myxobacterial steroids and cloning of the first bacterial 2,3(S)-oxidosqualene cyclase from the my
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