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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,782 - 1,786 (5page)
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초록· 키워드
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A new improved PCR method has been developed for the rapid, reliable, and sensitive detection of Venturia nashicola, a destructive pathogen of scab disease in Japanese pear. The translation elongation factor-1 alpha gene-derived PCR primers specifically amplified a 257-bp-sized DNA band of the target gene from the genomic DNA of V. nashicola. No amplicon was produced from the genomic DNA of other Venturia spp. and reference fungal species tested. With the high detection limit of 10 fg DNA content, our real-time method could be used for the quarantine inspection and field monitoring of V. nashicola.
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참고문헌 (11)
참고문헌 신청
Carbone I / 1999 / A method for designing primer sets for speciation studies in filamentous ascomycetes / Mycologia 91 : 553 ~ 556
Cho EK / 1985 / The causal organism of pear scab in Korea / Korean J. Mycol 13 : 263 ~ 265
Kim SH / 1999 / Rapidd etection of Ophiostoma piceae and O. quercus in stained wood by PCR / Appl. Environ. Microbiol 65 : 287 ~ 290
Le Cam B / 2001 / Specific polymerase chain reaction identification of Venturia nashicola using internally transcribed spacer region in the ribosomal DNA / Phytopathology 91
Livak KJ / 2001 / Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method / Methods 25 : 402 ~ 408
Cho EK / 1985 / The causal organism of pear scab in Korea / Korean J. Mycol 13 : 263 ~ 265
Kim SH / 1999 / Rapidd etection of Ophiostoma piceae and O. quercus in stained wood by PCR / Appl. Environ. Microbiol 65 : 287 ~ 290
Le Cam B / 2001 / Specific polymerase chain reaction identification of Venturia nashicola using internally transcribed spacer region in the ribosomal DNA / Phytopathology 91
Livak KJ / 2001 / Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCT method / Methods 25 : 402 ~ 408
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