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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,819 - 1,826 (8page)
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Poly(ε-L-lysine) (ε-PL) is a novel bioactive polymer secreted by filamentous bacteria. Owing to lack of a genetic system for most ε-PL-producing strains, very little research on enhancing ε-PL biosynthesis by genetic manipulation has been reported. In this study, an effective genetic system was established via intergeneric conjugal transfer for Streptomyces albulus PD-1, a famous ε-PL-producing strain. Using the established genetic system, the Vitreoscilla hemoglobin (VHb) gene was integrated into the chromosome of S. albulus PD-1 to alleviate oxygen limitation and to enhance the biosynthesis of ε-PL in submerged fermentation. Ultimately, the production of ε-PL increased from 22.7 g/l to 34.2 g/l after fed-batch culture in a 5 L bioreactor. Determination of the oxygen uptake rate, transcriptional level of ε-PL synthetase gene, and ATP level unveiled that the expression of VHb in S. albulus PD-1 enhanced ε-PL biosynthesis by improving respiration and ATP supply. To the best of our knowledge, this is the first report on enhancing ε-PL production by chromosomal integration of the VHb gene in an ε-PL-producing strain, and it will open a new avenue for ε-PL production.
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참고문헌 (23)
참고문헌 신청
Bankar SB / 2013 / Panorama of poly-ε-lysine / RSC Adv 3 : 8586 ~ 8603
Bankar, Sandip B. / 2011 / Improved Poly-ε-Lysine Biosynthesis Using Streptomyces noursei NRRL 5126 by Controlling Dissolved Oxygen During Fermentation / Journal of Microbio
Cheng YQ / 2002 / Identification and localization of the gene cluster encoding biosynthesis of the antitumor macrolactam leinamycin in Streptomyces atroolivaceus S-140 / J. B
Chi P / 2009 / Vitreoscilla hemoglobin aids respiration under hypoxic conditions in its native host / Microbiol. Res 164 : 267 ~ 275
Flett F / 1997 / High efficiency interg eneric c onjug al t ransfer o f plasmid D NA f rom Escherichia coli to methyl DNA-restricting streptomycetes / FEMS Microbiol. Lett 15
Bankar, Sandip B. / 2011 / Improved Poly-ε-Lysine Biosynthesis Using Streptomyces noursei NRRL 5126 by Controlling Dissolved Oxygen During Fermentation / Journal of Microbio
Cheng YQ / 2002 / Identification and localization of the gene cluster encoding biosynthesis of the antitumor macrolactam leinamycin in Streptomyces atroolivaceus S-140 / J. B
Chi P / 2009 / Vitreoscilla hemoglobin aids respiration under hypoxic conditions in its native host / Microbiol. Res 164 : 267 ~ 275
Flett F / 1997 / High efficiency interg eneric c onjug al t ransfer o f plasmid D NA f rom Escherichia coli to methyl DNA-restricting streptomycetes / FEMS Microbiol. Lett 15
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