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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제11호
- 발행연도
- 2015.1
- 수록면
- 1,810 - 1,818 (9page)
이용수
초록· 키워드
오류제보하기
For the synthesis of various pharmaceuticals, chiral alcohols are useful intermediates. Among them, (R)-ethyl-4-chloro-3-hydroxybutanoate ((R)-ECHB) is an important building block for the synthesis of L-carnitine. (R)-ECHB is produced from ethyl-4-chloro-3-oxobutanoate (ECOB) by a reductase-mediated, enantioselective reduction reaction. The Saccharomyces cerevisiae YOL151W reductase that is expressed in Escherichia coli cells exhibited an enantioselective reduction reaction toward ECOB. By virtue of the C-terminal His-tag, the YOL151W reductase was purified from the cell-free extract using Ni2+-NTA column chromatography and immobilized onto Ni2+-magnetic microparticles. The physical properties of the immobilized reductase (Imm-Red) were measured using electron microscopy, a magnetic property measurement system, and a zeta potential system; the average size of the particles was approximately 1 μm and the saturated magnetic value was 31.76 emu/g. A neodymium magnet was used to recover the immobilized enzyme within 2 min. The Imm-Red showed an optimum temperature at 45oC and an optimum pH at 6.0. In addition, Bacillus megaterium glucose dehydrogenase (GDH) was produced in the E. coli cells and was used in the coupling reaction to regenerate the NADPH cofactor. The reduction/oxidation coupling reaction composed of the Imm-Red and GDH converted 20 mM ECOB exclusively into (R)- ECHB with an e.e.p value of 98%.
목차
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참고문헌 (22)
참고문헌 신청
Aragozzini F / 1992 / Biocatalytic, enantioselective preparations of (R)- and (S)-ethyl 4-chloro-3-hydroxybutanoate, a useful chiral synthon / Biocatalysis 5 : 325 ~ 332
Bajaj P / 2014 / Stabilization studies on bacterially produced human paraoxonase 1 for improving its shelf life / Appl. Biochem. Biotechnol 172 : 3798 ~ 3809
Bhattacharyya MS / 2010 / Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone / Bioresour. Tech
Chang BS / 1995 / Enzyme thermostabilization by bovine serum albumin and other proteins: evidence for hydrophobic interactions / Biotechnol. Appl. Biochem 22 : 203 ~ 214
Choi YH / 2010 / Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity / Appl. Microbiol. Biotechnol
Bajaj P / 2014 / Stabilization studies on bacterially produced human paraoxonase 1 for improving its shelf life / Appl. Biochem. Biotechnol 172 : 3798 ~ 3809
Bhattacharyya MS / 2010 / Immobilization of intracellular carbonyl reductase from Geotrichum candidum for the stereoselective reduction of 1-naphthyl ketone / Bioresour. Tech
Chang BS / 1995 / Enzyme thermostabilization by bovine serum albumin and other proteins: evidence for hydrophobic interactions / Biotechnol. Appl. Biochem 22 : 203 ~ 214
Choi YH / 2010 / Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity / Appl. Microbiol. Biotechnol
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