지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제26권 제11호
- 발행연도
- 2016.1
- 수록면
- 1,863 - 1,870 (8page)
이용수
초록· 키워드
오류제보하기
Antimicrobial peptides/proteins (AMPs) are present in all types of organisms, from microbes and plants to vertebrates and invertebrates such as insects. The grasshopper Oxya chinensis sinuosa is an insect species that is widely consumed around the world for its broad medicinal value. However, the lack of available genetic information for this species is an obstacle to understanding the full potential of its AMPs. Analysis of the O. chinensis sinuosa transcriptome and expression profile is essential for extending the available genetic information resources. In this study, we determined the whole-body transcriptome of O. chinensis sinuosa and analyzed the potential AMPs induced by bacterial immunization. A high-throughput RNA-Seq approach generated 94,348 contigs and 66,555 unigenes. Of these unigenes, 36,032 (54.14%) matched known proteins in the NCBI database in a BLAST search. Functional analysis demonstrated that 38,219 unigenes were clustered into 5,499 gene ontology terms. In addition, 26 cDNAs encoding novel AMPs were identified by an in silico approach using public databases. Our transcriptome dataset and AMP profile greatly improve our understanding of O. chinensis sinuosa genetics and provide a huge number of gene sequences for further study, including genes of known importance and genes of unknown function.
목차
등록된 정보가 없습니다.
참고문헌 (26)
참고문헌 신청
Azam I / 2015 / Evaluating insects as bioindicators of heavy metal contamination and accumulation near industrial area of Gujrat, Pakistan / Biomed. Res. Int 2015 : 942751 ~
Badisco L / 2011 / Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database / PLoS One 6 : e17274
Badman J / 2007 / Grasshoppers in research and education: methods for maintenance and production / Lab Anim. (NY) 36 : 27 ~
Chen S / 2010 / De novo analysis of transcriptome dynamics in the migratory locust during the development of phase traits / PLoS One 5 : e15633 ~
Cherniack EP / 2010 / Bugs as drugs, Part 1: Insects: the “new”alternative medicine for the 21st century / Altern. Med. Rev 15 : 124 ~ 135
Badisco L / 2011 / Transcriptome analysis of the desert locust central nervous system: production and annotation of a Schistocerca gregaria EST database / PLoS One 6 : e17274
Badman J / 2007 / Grasshoppers in research and education: methods for maintenance and production / Lab Anim. (NY) 36 : 27 ~
Chen S / 2010 / De novo analysis of transcriptome dynamics in the migratory locust during the development of phase traits / PLoS One 5 : e15633 ~
Cherniack EP / 2010 / Bugs as drugs, Part 1: Insects: the “new”alternative medicine for the 21st century / Altern. Med. Rev 15 : 124 ~ 135
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