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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제2호
- 발행연도
- 2017.1
- 수록면
- 342 - 349 (8page)
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초록· 키워드
오류제보하기
Polylactic acid (PLA) has been highlighted as an alternative renewable polymer for the replacement of petroleum-based plastic materials, and is considered to be biodegradable. On the other hand, the biodegradation of PLA by terminal degraders, such as microorganisms, requires a lengthy period in the natural environment, and its mechanism is not completely understood. PLA biodegradation studies have been conducted using mainly undefined mixed cultures, but only a few bacterial strains have been isolated and examined. For further characterization of PLA biodegradation, in this study, the PLA-degrading bacteria from digester sludge were isolated and identified using a polymer film-based screening method. The enrichment of sludge on PLA granules was conducted with the serial transference of a subculture into fresh media for 40 days, and the attached biofilm was inoculated on a PLA film on an agar plate. 3D optical microscopy showed that the isolates physically degraded the PLA film due to bacterial degradation. 16S rRNA gene sequencing identified the microbial colonies to be Pseudomonas sp. MYK1 and Bacillus sp. MYK2. The two isolates exhibited significantly higher specific gas production rates from PLA biodegradation compared with that of the initial sludge inoculum.
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참고문헌 (23)
참고문헌 신청
Akutsu-Shigeno Y / 2003 / Cloning and sequencing of a poly (DL-lactic acid)depolymerase gene from Paenibacillus amylolyticus strain TB-13 and its functional expression in Es
Bettmann H / 1984 / Degradation of phenol by polymer entrapped microorganisms / Appl. Microbiol. Biotechnol 20 : 285 ~ 290
Bikiaris DN. / 2013 / Nanocomposites of aliphatic polyesters:an overview of the effect of different nanofillers on enzymatic hydrolysis and biodegradation of polyesters / Pol
Kim JR / 2007 / Power generation using different cation, anion, and ultrafiltration membranes in microbial fuel cells / Environ. Sci. Technol 41 : 1004 ~ 1009
Lane DJ / 1985 / Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses / Proc. Natl. Acad. Sci. USA 82 : 6955 ~ 6959
Bettmann H / 1984 / Degradation of phenol by polymer entrapped microorganisms / Appl. Microbiol. Biotechnol 20 : 285 ~ 290
Bikiaris DN. / 2013 / Nanocomposites of aliphatic polyesters:an overview of the effect of different nanofillers on enzymatic hydrolysis and biodegradation of polyesters / Pol
Kim JR / 2007 / Power generation using different cation, anion, and ultrafiltration membranes in microbial fuel cells / Environ. Sci. Technol 41 : 1004 ~ 1009
Lane DJ / 1985 / Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses / Proc. Natl. Acad. Sci. USA 82 : 6955 ~ 6959
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