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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제11호
- 발행연도
- 2018.1
- 수록면
- 1,850 - 1,858 (9page)
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초록· 키워드
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Glucosamine (GlcN) is widely used in the nutraceutical and pharmaceutical industries. Currently, GlcN is mainly produced by traditional multistep chemical synthesis and acid hydrolysis, which can cause severe environmental pollution, require a long prodution period but a lower yield. The aim of this work was to develop a whole-cell biocatalytic process for the environment-friendly synthesis of glucosamine (GlcN) from N-acetylglucosamine (GlcNAc). We constructed a recombinant Escherichia coli and Bacillus subtilis strains as efficient whole-cell biocatalysts via expression of diacetylchitobiose deacetylase (Dacph) from Pyrococcus furiosus. Although both strains were biocatalytically active, the performance of B. subtilis was better. To enhance GlcN production, optimal reaction conditions were found: B. subtilis whole-cell biocatalyst 18.6 g/l, temperature 40°C, pH 7.5, GlcNAc concentration 50 g/l and reaction time 3 h. Under the above conditions, the maximal titer of GlcN was 35.3 g/l, the molar conversion ratio was 86.8% in 3-L bioreactor. This paper shows an efficient biotransformation process for the biotechnological production of GlcN in B. subtilis that is more environmentally friendly than the traditional multistep chemical synthesis approach. The biocatalytic process described here has the advantage of less environmental pollution and thus has great potential for largescale production of GlcN in an environment-friendly manner.
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참고문헌 (23)
참고문헌 신청
Chen JK / 2010 / N-acetylglucosamine : production and applications / Marine Drugs 8:2493~2516
Nakamura H. / 2011 / Application of glucosamine on human disease—Osteoarthritis / Carbohydr. Polym 84:835~839
Hungerford DS / 2003 / Glucosamine and chondroitin sulfate are effective in the management of osteoarthritis / J. Arthroplasty 18:5~9
Towheed TE. / 2003 / Current status of glucosamine therapy in osteoarthritis / Arthritis Rheum 49:601~604
Sitanggang AB / 2010 / Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742 / Bioresour. Technol 101:3595~3601
Nakamura H. / 2011 / Application of glucosamine on human disease—Osteoarthritis / Carbohydr. Polym 84:835~839
Hungerford DS / 2003 / Glucosamine and chondroitin sulfate are effective in the management of osteoarthritis / J. Arthroplasty 18:5~9
Towheed TE. / 2003 / Current status of glucosamine therapy in osteoarthritis / Arthritis Rheum 49:601~604
Sitanggang AB / 2010 / Effect of pellet size and stimulating factor on the glucosamine production using Aspergillus sp. BCRC 31742 / Bioresour. Technol 101:3595~3601
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