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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제7호
- 발행연도
- 2015.1
- 수록면
- 1,101 - 1,107 (7page)
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The role of CRE1 in a thermophilic fungus, Myceliophthora thermophila ATCC42464, was studied using RNA interference. In the cre1-silenced strain C88, the filter paper hydrolyzing activity and β-1,4-endoglucanase activity were 3.76-, and 1.31-fold higher, respectively, than those in the parental strain when the strains were cultured in inducing medium for 6 days. The activities of β-1,4-exoglucanase and cellobiase were 2.64-, and 5.59-fold higher, respectively, than those in the parental strain when the strains were cultured for 5 days. Quantitative reverse-transcription polymerase chain reaction showed that the gene expression of egl3, cbh1, and cbh2 was significantly increased in transformant C88 compared with the wild-type strain. Therefore, our findings suggest the feasibility of improving cellulase production by modifying the regulator expression, and an attractive approach to increasing the total cellulase productivity in thermophilic fungi.
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참고문헌 (23)
참고문헌 신청
An-Na Li / 2011 / Cloning, Expression, and Characterization of Serine Protease from Thermophilic Fungus Thermoascus aurantiacus var. levisporus / The Journal of Microbiology
Antonieto AC / 2014 / Defining the genome-wide role of CRE1during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis / Fungal Genet. Biol 73 : 93 ~ 103
Battle A / 2014 / Impact of regulatory variation from RNA to protein / Science : 1260793 ~
Berka RM / 2011 / Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris / Nat. Biotechnol 29 : 922 ~ 92
Brummelkamp TR / 2002 / A system for stable expression of short interfering RNAs in mammalian cells / Science 296 : 550 ~ 553
Antonieto AC / 2014 / Defining the genome-wide role of CRE1during carbon catabolite repression in Trichoderma reesei using RNA-Seq analysis / Fungal Genet. Biol 73 : 93 ~ 103
Battle A / 2014 / Impact of regulatory variation from RNA to protein / Science : 1260793 ~
Berka RM / 2011 / Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris / Nat. Biotechnol 29 : 922 ~ 92
Brummelkamp TR / 2002 / A system for stable expression of short interfering RNAs in mammalian cells / Science 296 : 550 ~ 553
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