지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,291 - 1,298 (8page)
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초록· 키워드
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Five different polymer nanofibers, namely, polyaniline nanofiber (PANI), magnetically separable polyaniline nanofiber (PAMP), magnetically separable DEAE cellulose fiber (DEAE), magnetically separable CM cellulose fiber (CM), and polystyrene nanofiber (PSNF), have been used for the immobilization of lactase (E.C. 3.2.1.23). Except for CM and PSNF, three polymers showed great properties. The catalytic activities (kcat) of the free, PANI, PAMP, and magnetic DEAE-cellulose were determined to be 4.0, 2.05, 0.59, and 0.042 mM/min·mg protein, respectively. The lactase immobilized on DEAE, PANI, and PAMP showed improved stability and recyclability. PANI- and PAMP-lactase showed only a 0-3% decrease in activity after 3 months of vigorous shaking conditions (200 rpm) and at room temperature (25oC). PANI-, PAMP-, and DEAE-lactase showed a high percentage of conversion (100%, 47%, and 12%) after a 1 h lactose hydrolysis reaction. The residual activities of PANI-, PAMP-, and DEAE-lactase after 10 times of recycling were 98%, 96%, and 97%, respectively.
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등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
[학술지
Al-Muftah AE / 2005 / Effects of internal mass transfer and product inhibition on a simulated immobilized enzyme-catalyzed reactor for lactose hydrolysis / Biochem. Eng. J 23
Al-Muftah AE / 2005 / Effects of simultaneous internal and external mass transfer and product inhibition on immobilized enzyme-catalyzed reactor / Biochem. Eng. J 27 : 167 ~
Ansari SA / 2011 / Immobilization of Kluyveromyces lactis β-galactosidase on concanavalin A layered aluminium oxide nanoparticles ? Its future aspects in biosensor applicati
Bayramoglu G / 2007 / Immobilization of β-galactosidase onto magnetic poly(GMA-MMA) beads for hydrolysis of lactose in bed reactor / Catal. Commun 8 : 1094 ~ 1101
Al-Muftah AE / 2005 / Effects of internal mass transfer and product inhibition on a simulated immobilized enzyme-catalyzed reactor for lactose hydrolysis / Biochem. Eng. J 23
Al-Muftah AE / 2005 / Effects of simultaneous internal and external mass transfer and product inhibition on immobilized enzyme-catalyzed reactor / Biochem. Eng. J 27 : 167 ~
Ansari SA / 2011 / Immobilization of Kluyveromyces lactis β-galactosidase on concanavalin A layered aluminium oxide nanoparticles ? Its future aspects in biosensor applicati
Bayramoglu G / 2007 / Immobilization of β-galactosidase onto magnetic poly(GMA-MMA) beads for hydrolysis of lactose in bed reactor / Catal. Commun 8 : 1094 ~ 1101
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