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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제8호
- 발행연도
- 2015.1
- 수록면
- 1,227 - 1,233 (7page)
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초록· 키워드
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Vascular endothelial growth factor (VEGF) plays a key role in angiogenesis through binding to its specific receptors, which mainly occurs to VEGF receptor 2 (VEGFR-2), a kinase insert domain-containing receptor. Therefore, the disruption of VEGFR-2 signaling provides a promising therapeutic approach for the treatment of cancer by inhibiting abnormal or tumorinduced angiogenesis. To explore this potential, we expressed the catalytic domain of VEGFR- 2 (VEGFR-2-CD) as a soluble active kinase in Escherichia coli. The recombinant protein was purified and the VEGFR-2-CD activity was investigated. The obtained VEGFR-2-CD showed autophosphorylation activity and phosphate transfer activity comparable to the commercial enzyme. Furthermore, the IC50 value of known VEGFR-2 inhibitor was determined using the purified VEGFR-2-CD. These results indicated a possibility for functional and economical VEGFR-2-CD expression in E. coli to use for inhibitor screening.
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참고문헌 (22)
참고문헌 신청
Arora A / 2005 / Role of tyrosine kinase inhibitors in cancer therapy / J. Pharmacol. Exp. Ther 315 : 971 ~ 979
Baneyx F. / 1999 / Recombinant protein expression in Escherichia coli / Curr. Opin. Biotechnol 10 : 411 ~ 421
Brignola PS / 2002 / Comparison of the biochemical and kinetic properties of the type 1 receptor tyrosine kinase intracellular domains. Demonstration of differential sensitiv
Carvalho JEF / 2007 / Vascular endothelial growth factor (VEGF) in autoimmune diseases / J. Clin. Immunol 27 : 246 ~ 256
Doughervermazen M / 1994 / Biological activity and phosphorylation sites of the bacterially expressed cytosolic domain of the KDR VEGF-receptor / Biochem. Biophys. Res. Commu
Baneyx F. / 1999 / Recombinant protein expression in Escherichia coli / Curr. Opin. Biotechnol 10 : 411 ~ 421
Brignola PS / 2002 / Comparison of the biochemical and kinetic properties of the type 1 receptor tyrosine kinase intracellular domains. Demonstration of differential sensitiv
Carvalho JEF / 2007 / Vascular endothelial growth factor (VEGF) in autoimmune diseases / J. Clin. Immunol 27 : 246 ~ 256
Doughervermazen M / 1994 / Biological activity and phosphorylation sites of the bacterially expressed cytosolic domain of the KDR VEGF-receptor / Biochem. Biophys. Res. Commu
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