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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 356 - 362 (7page)
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초록· 키워드
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Ceramide is important not only for the maintenance of the barrier function of the skin but also for the waterbinding capacity of the stratum corneum. Although the exact role of ceramide in the human skin is not fully understood, ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries. Compared with other microorganisms, yeast is more suitable for the production of ceramide because yeast grows fast and is non-toxic. However, production of ceramide from yeast has not been widely studied and most work in this area has been carried out using Saccharomyces cerevisiae.
Regulating the genes that are involved in sphingolipid synthesis is necessary to increase ceramide production. In this study, we investigated the effect of the genes involved in the synthesis of ceramide, lcb1, lcb2, tsc10, lac1, lag1,and sur2, on ceramide production levels. The genes were cloned into pYES2 high copy number vectors. S. cerevisiae was cultivated on YPDG medium at 30oC. Ceramide was purified from the cell extracts by solvent extraction and the ceramide content was analyzed by HPLC using ELSD.
The maximum production of ceramide (9.8 mg ceramide/g cell) was obtained when the tsc10 gene was amplified by the pYES2 vector. Real-time RT-PCR analysis showed that the increase in ceramide content was proportional to the increase in the tsc10 gene expression level, which was 4.56 times higher than that of the control strain.
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참고문헌 (27)
참고문헌 신청
/ / Saccharomyces Genome Database
Bustin, S. A / 2000 / Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays / J. Mol. Endocrinol 25 : 169 ~ 193
Dickson, R. C / 1997 / Sphingolipids are potential heat stress signals in Saccharomyces / J. Biol. Chem 272 : 30196 ~ 30200
Dickson, R. C / 1999 / Yeast sphingolipids / Biochim. Biophys. Acta 1426 : 347 ~ 357
Dickson, R. C / 2002 / Sphingolipid functions in Saccharomyces cerevisiae / Biochim. Biophys. Acta 1583 : 13 ~ 25
Bustin, S. A / 2000 / Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays / J. Mol. Endocrinol 25 : 169 ~ 193
Dickson, R. C / 1997 / Sphingolipids are potential heat stress signals in Saccharomyces / J. Biol. Chem 272 : 30196 ~ 30200
Dickson, R. C / 1999 / Yeast sphingolipids / Biochim. Biophys. Acta 1426 : 347 ~ 357
Dickson, R. C / 2002 / Sphingolipid functions in Saccharomyces cerevisiae / Biochim. Biophys. Acta 1583 : 13 ~ 25
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