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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제5호
- 발행연도
- 2015.1
- 수록면
- 732 - 737 (6page)
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We present here an in silico search of fungal sterol-esterase/lipase and bacterial depolymerase sequences from environmental metagenomes. Both enzyme types contain the α/β-hydrolase protein fold. Analysis of DNA conserved motifs, protein homology search, phylogenetic analysis, and protein 3D modeling have been used, and the efficiency of these screening strategies is discussed. The presence of bacterial genes in the metagenomes was higher than those from fungi, and the sequencing depth of the metagenomes seemed to be crucial to allow finding enough diversity of enzyme sequences. As a result, a novel putative PHAdepolymerase is described.
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참고문헌 (25)
참고문헌 신청
Altschul SF / 1990 / Basic local alignment search tool / J. Mol. Biol 215 : 403 ~ 410
Bailey TL / 2009 / MEME SUITE: tools for motif discovery and searching / Nucleic Acids Res 37 : W202 ~ W208
Barriuso J / 2008 / Effect of inoculation with putative PGPR isolated from Pinus sp. on Pinus pinea growth, mycorrhization and rhizosphere microbial communities / J. Appl. Mi
Barriuso J / 2011 / Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows / BMC Bioinformatics 14 : 473 ~
Barriuso J / 2013 / Fungal geno mes mining to discover novel sterol esterases and lipases as catalysts / BMC Genomics 18 : 712 ~
Bailey TL / 2009 / MEME SUITE: tools for motif discovery and searching / Nucleic Acids Res 37 : W202 ~ W208
Barriuso J / 2008 / Effect of inoculation with putative PGPR isolated from Pinus sp. on Pinus pinea growth, mycorrhization and rhizosphere microbial communities / J. Appl. Mi
Barriuso J / 2011 / Estimation of bacterial diversity using next generation sequencing of 16S rDNA: a comparison of different workflows / BMC Bioinformatics 14 : 473 ~
Barriuso J / 2013 / Fungal geno mes mining to discover novel sterol esterases and lipases as catalysts / BMC Genomics 18 : 712 ~
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