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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제10호
- 발행연도
- 2015.1
- 수록면
- 1,714 - 1,727 (14page)
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This work describes a new strategy for optimal design of Multiplex-PCR primer sequences. The process is based on the Particle Swarm Optimization-Simplex algorithm (Mult-PSOS). Diverging from previous solutions centered on heuristic tools, the Mult-PSOS is selfconfigured because it does not require the definition of the algorithm’s initial search parameters. The successful performance of this method was validated in vitro using Multiplex- PCR assays. For this validation, seven gene sequences of the most prevalent bacteria implicated in urinary tract infections were taken as DNA targets. The in vitro tests confirmed the good performance of the Mult-PSOS, with respect to infectious disease diagnosis, in the rapid and efficient selection of the optimal oligonucleotide sequences for Multiplex-PCRs. The predicted sequences allowed the adequate amplification of all amplicons in a single step (with the correct amount of DNA template and primers), reducing significantly the need for trial and error experiments. In addition, owing to its independence from the initial selection of the heuristic constants, the Mult-PSOS can be employed by non-expert users in computational techniques or in primer design problems.
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참고문헌 (24)
참고문헌 신청
Abd-Elsalam KA / 2003 / Bioinformatic tools and guideline for PCR primer design / Afr. J. Biotechnol 2 : 91 ~ 95
Abdeldaim GM / 2010 / Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae
Allawi HT / 1997 / Thermodynamics and NMR of internal GT mismatches in DNA / Biochemistry 36 : 10581 ~ 10594
Altschul SF / 1990 / Basic local alignment search tool / J. Mol. Biol 215 : 403 ~ 410
Amghalia E / 2009 / Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in Staphylococcus aureus isolated from Malaysian hospitals / Res
Abdeldaim GM / 2010 / Multiplex quantitative PCR for detection of lower respiratory tract infection and meningitis caused by Streptococcus pneumoniae, Haemophilus influenzae
Allawi HT / 1997 / Thermodynamics and NMR of internal GT mismatches in DNA / Biochemistry 36 : 10581 ~ 10594
Altschul SF / 1990 / Basic local alignment search tool / J. Mol. Biol 215 : 403 ~ 410
Amghalia E / 2009 / Multiplex PCR assays for the detection of clinically relevant antibiotic resistance genes in Staphylococcus aureus isolated from Malaysian hospitals / Res
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