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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제12호
- 발행연도
- 2015.1
- 수록면
- 2,024 - 2,033 (10page)
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초록· 키워드
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A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activity increased in the presence of 30% methanol and, remarkably, increased as well to 154.6% in the presence of Ca2+. Lip7-3 preferred pnitrophenyl octanoate (C8) as a substrate and exhibited broad specificity for short- to longchain fatty acid esters. Additionally, Lip7-3 showed a low degree of enantioselectivity for an S-enantiomer (e.g., (S)-methyl-3-hydroxy-2-methylpropionate). It efficiently hydrolyzed glyceryl tributyrate, but did not hydrolyze glyceryl trioleate, fish oil, or olive oil. Its substrate specificity and activation by the solvent might offer a merit to the biotechnological enzyme applications like transesterification in the production of biodiesel.
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참고문헌 (24)
참고문헌 신청
Akbulut N / 2013 / Improved activity and thermostability of Bacillus pumilus lipase by directed evolution / J. Biotechnol 164 : 123 ~ 129
Arpigny JL / 1999 / Bacterial lipolytic enzymes:classification and properties / Biochem. J 343 : 177 ~ 183
Bae SY / 2013 / Characterization and immobilization of a novel SGNH hydrolase (Est24) from Sinorhizobium meliloti / Appl. Microbiol. Biotechnol 97 : 1637 ~ 1647
Charbonneau DM / 2013 / Role of key salt bridges in thermostability of G. thermodenitrificans EstGtA2:distinctive patterns within the new bacterial lipolytic enzyme family XV
Dartois V / 1992 / Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168 / Biochim. Biophys. Acta 1131 : 253 ~ 260
Arpigny JL / 1999 / Bacterial lipolytic enzymes:classification and properties / Biochem. J 343 : 177 ~ 183
Bae SY / 2013 / Characterization and immobilization of a novel SGNH hydrolase (Est24) from Sinorhizobium meliloti / Appl. Microbiol. Biotechnol 97 : 1637 ~ 1647
Charbonneau DM / 2013 / Role of key salt bridges in thermostability of G. thermodenitrificans EstGtA2:distinctive patterns within the new bacterial lipolytic enzyme family XV
Dartois V / 1992 / Cloning, nucleotide sequence and expression in Escherichia coli of a lipase gene from Bacillus subtilis 168 / Biochim. Biophys. Acta 1131 : 253 ~ 260
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