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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제8호
- 발행연도
- 2018.1
- 수록면
- 1,376 - 1,383 (8page)
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초록· 키워드
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The hepatitis B virus (HBV) envelope contains small (S), middle (M), and large (L) proteins. PreS1 of the L protein contains a receptor-binding motif crucial for HBV infection. This motif is highly conserved among 10 HBV genotypes (A–J), making it a potential target for the prevention of HBV infection. In this study, we successfully generated a neutralizing human monoclonal antibody (mAb), 1A8 (IgG1), that recognizes the receptor-binding motif of preS1 using a phage-displayed human synthetic Fab library. Analysis of the antigen-binding activity of 1A8 for different genotypes indicated that it can specifically bind to the preS1 of major HBV genotypes (A–D). Based on Bio-Layer interferometry, the affinity (KD) of 1A8 for the preS1 of genotype C was 3.55 nM. 1A8 immunoprecipitated the hepatitis B virions of genotypes C and D. In an in vitro neutralization assay using HepG2 cells overexpressing the cellular receptor sodium taurocholate cotransporting polypeptide, 1A8 effectively neutralized HBV infection with genotype D. Taken together, the results suggest that 1A8 may neutralize the four HBV genotypes. Considering that genotypes A–D are most prevalent, 1A8 may be a neutralizing human mAb with promising potential in the prevention and treatment of HBV infection.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
WHO / 2017 / Global Hepatitis Report 2017
Sunbul M / 2014 / Hepatitis B virus genotypes: global distribution and clinical importance / World J. Gastroenterol. 20:5427
Ganem D / 2004 / Hepatitis B virus infection - natural history and clinical consequences / N. Engl. J. Med. 350:1118~1129
Huang C-F / 2006 / The immune response induced by hepatitis B virus principal antigens / Cell. Mol. Immunol. 3:97~106
Schulze A / 2010 / Fine mapping of pre-S sequence requirements for hepatitis B virus large envelope protein-mediated receptor interaction / J. Virol. 84:1989~2000
Sunbul M / 2014 / Hepatitis B virus genotypes: global distribution and clinical importance / World J. Gastroenterol. 20:5427
Ganem D / 2004 / Hepatitis B virus infection - natural history and clinical consequences / N. Engl. J. Med. 350:1118~1129
Huang C-F / 2006 / The immune response induced by hepatitis B virus principal antigens / Cell. Mol. Immunol. 3:97~106
Schulze A / 2010 / Fine mapping of pre-S sequence requirements for hepatitis B virus large envelope protein-mediated receptor interaction / J. Virol. 84:1989~2000
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