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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제8호
- 발행연도
- 2009.1
- 수록면
- 781 - 786 (6page)
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Small interfering synthetic double-stranded RNA (siRNA)
was applied to suppress the expression of the human cytotoxic-
T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) gene
transformed in transgenic rice cell cultures. The sequence
of the 21-nucleotide siRNA was deliberately designed and
synthesized with overhangs to inactivate the expression of
hCTLA4Ig. The chemically synthesized siRNA duplex was
combined with polyethyleneimine (PEI) at a mass ratio of
1:10 (0.33 μg siRNA:3.3 μg PEI) to produce complexes.
The siRNA complexes (siRNA+PEI) were labeled with
Cy3 in order to subsequently confirm the delivery by
fluorescent microscopy. In addition, the cells were treated
with sonoporation at 40 kHz and 419 W for 90 s to improve
the delivery. The siRNA complexes alone inhibited the
expression of hCTLA4Ig to 45% compared with control. The
siRNA complexes delivered with sonoporation downregulated
the production of hCTLA4Ig to 73%. Therefore, we
concluded that the delivery of siRNA complexes into plant
cells could be enhanced successfully by sonoporation.
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참고문헌 (26)
참고문헌 신청
[학술지(정기간행물)] -
CHEON, SU-HWAN / 2007 / Effects of Silkworm Hemolymph on Cell Viability and hCTLA4Ig Productionin Transgenic Rice Cell Suspension Cultures / Journal of Microbiology and Biote
Chen, T. L. / 2004 / Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures / Transgenic Res 13 : 499 ~ 510
Duxbury, M. S. / 2004 / RNA Interference: A practical approach / J. Surg. Res 117 : 339 ~ 344
Elouahabi, A. / 2005 / Formation and intracellular trafficking of lipoplexes and polyplexes / Mol. Ther 11 : 336 ~ 347
CHEON, SU-HWAN / 2007 / Effects of Silkworm Hemolymph on Cell Viability and hCTLA4Ig Productionin Transgenic Rice Cell Suspension Cultures / Journal of Microbiology and Biote
Chen, T. L. / 2004 / Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures / Transgenic Res 13 : 499 ~ 510
Duxbury, M. S. / 2004 / RNA Interference: A practical approach / J. Surg. Res 117 : 339 ~ 344
Elouahabi, A. / 2005 / Formation and intracellular trafficking of lipoplexes and polyplexes / Mol. Ther 11 : 336 ~ 347
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