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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제2호
- 발행연도
- 2015.1
- 수록면
- 227 - 233 (7page)
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초록· 키워드
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Two recombinant arabinosyl hydrolases, α-L-arabinofuranosidase from Geobacillus sp. KCTC 3012 (GAFase) and endo-(1,5)-α-L-arabinanase from Bacillus licheniformis DSM13 (BlABNase), were overexpressed in Escherichia coli, and their synergistic modes of action against sugar beet (branched) arabinan were investigated. Whereas GAFase hydrolyzed 35.9% of L-arabinose residues from sugar beet (branched) arabinan, endo-action of BlABNase released only 0.5% of L-arabinose owing to its extremely low accessibility towards branched arabinan. Interestingly, the simultaneous treatment of GAFase and BlABNase could liberate approximately 91.2% of L-arabinose from arabinan, which was significantly higher than any single exo-enzyme treatment (35.9%) or even stepwise exo- after endo-enzyme treatment (75.5%). Based on their unique modes of action, both exo- and endo-arabinosyl hydrolases can work in concert to catalyze the hydrolysis of arabinan to L-arabinose. At the early stage in arabinan degradation, exo-acting GAFase could remove the terminal arabinose branches to generate debranched arabinan, which could be successively hydrolyzed into arabinooligosaccharides via the endoaction of BlABNase. At the final stage, the simultaneous actions of exo- and endo-hydrolases could synergistically accelerate the L-arabinose production with high conversion yield.
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참고문헌 (22)
참고문헌 신청
Beldman G / 1993 / Degradation of arabinans by arabinanases from Aspergillus aculeatus and Aspergillus niger / Carbohydr. Polym 20 : 159 ~ 168
Bezalel L / 1993 / Characterization and delignification activity of a thermostable α-L-arabinofuranosidase from Bacillus stearothermophilus / Appl. Microbiol. Biotechnol 40
Canakci S / 2008 / Cloning, purification, and characterization of a thermostable α-L-arabinofuranosidase from Anoxybacillus kestanbolensis AC26Sari / Appl. Microbiol. Biotec
Greve LC / 1984 / α-LArabinofuranosidase from Ruminococcus albus 8: purification and possible role in hydrolysis of alfalfa cell wall / Appl. Environ. Microbiol 47 : 1135 ~
Hong MR / 2009 / Characterization of a thermostable endo-1,5-α-L-arabinanase from Caldicellulorsiruptor saccharolyticus / Biotechnol. Lett 31 : 1439 ~ 1443
Bezalel L / 1993 / Characterization and delignification activity of a thermostable α-L-arabinofuranosidase from Bacillus stearothermophilus / Appl. Microbiol. Biotechnol 40
Canakci S / 2008 / Cloning, purification, and characterization of a thermostable α-L-arabinofuranosidase from Anoxybacillus kestanbolensis AC26Sari / Appl. Microbiol. Biotec
Greve LC / 1984 / α-LArabinofuranosidase from Ruminococcus albus 8: purification and possible role in hydrolysis of alfalfa cell wall / Appl. Environ. Microbiol 47 : 1135 ~
Hong MR / 2009 / Characterization of a thermostable endo-1,5-α-L-arabinanase from Caldicellulorsiruptor saccharolyticus / Biotechnol. Lett 31 : 1439 ~ 1443
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