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초록· 키워드
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Soybean proteins are widely used for human and animal feed in the world. β-conglycinin protein exhibits poor nutritional and food processing properties and Kunitz trypsin inhibitor (KTI) protein is a main anti-nutritional factor in soybean seed. The objective of this research was to identify the inheritance of cgy1 gene and ti gene for the improvement of soybean cultivar with no KTI proteins and low amount of β-conglycinin. F2 population was made by crossing between “Gaechuck2ho”(α′-subunit present Cgy1Cgy1, KTI protein absent titi) and PI506876 (α′-subunit absent cgy1cgy1, KTI protein present TiTi) parent.
A total of 434 F2 seeds were obtained and analyzed for the segregation of α′-subunit protein and KTI protein using SDS-PAGE.
The segregation ratio of 3 : 1 for Cgy1 locus (310 Cgy1_ : 124 cgy1cgy1) and Ti locus (339 Ti_ : 95 titi) were observed.
Segregation ratios of 9 : 3 : 3 : 1 (241 Cgy1_Ti_: 69 Cgy1_titi: 98 cgy1cgy1Ti_: 26 cgy1cgy1titi) between Cgy1 gene and Ti gene in F2 seeds were also observed (χ2= 5.367, P = 0.10 - 0.20). This data showed that Cgy1 gene was inherited independently with the Ti gene in soybean. These results will be useful in breeding program for selecting the line that does not exhibit or lacks both α′-subunit protein and KTI protein in soybean.
목차
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참고문헌 (21)
참고문헌 신청
Birk Y / 1961 / Purification and some properties of a highly active trypsin and chymotrypsin from soybeans / Biochem. Acta 54 : 378 ~ 381
Chen Z / 1998 / Four genes affecting seed traits in soybeans map to linkage group F / The J. of Heredity 89 (3) : 211 ~ 215
Cregan PB / 1999 / An integrated genetic linkage map of the soybean / Crop Sci. 39 : 1464 ~ 1490
Frattali V / 1968 / Soybean inhibitor. I. Separationssome properties of three inhibitors from commercial crudetrypsin inhibitors / Biochem. 7 : 521 ~ 530
Hajika M / 1998 / Dominant inheritance of a trait lacking β-conglycinin detected in a wild soybean line / Breeding Science 48 : 383 ~ 386
Chen Z / 1998 / Four genes affecting seed traits in soybeans map to linkage group F / The J. of Heredity 89 (3) : 211 ~ 215
Cregan PB / 1999 / An integrated genetic linkage map of the soybean / Crop Sci. 39 : 1464 ~ 1490
Frattali V / 1968 / Soybean inhibitor. I. Separationssome properties of three inhibitors from commercial crudetrypsin inhibitors / Biochem. 7 : 521 ~ 530
Hajika M / 1998 / Dominant inheritance of a trait lacking β-conglycinin detected in a wild soybean line / Breeding Science 48 : 383 ~ 386
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