지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26
produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy
locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its
transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction
(PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro
were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic
line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after
mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line
did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Bolar JP / 1999 / Factors affecting the transformation of ‘Marshall McIntosh’ apple by Agrobacterium tumefaciens / Plant Cell Tiss. Org. Cult. 55 : 31 ~ 38
Capone I / 1989 / Induction and growth properties of carrot roots with different complements of Agrobacterium rhizogenes T-DNA / Plant Mol Biol. 13 (1) : 43 ~ 52
Fladung M / 1990 / Transformation of diploid and tetraploid potato clones with the rolC gene of Agrobacterium rhizogenes and characterization of transgenic plants / Plant Bre
Gardner N / 2006 / Differential expression of rolC results in unique plant phenotypes / J. Amer. Soc. Hort. Sci. 131 (1) : 82 ~ 88
Han SG / 2004 / Figure of freezing injury in apple trees on M.9 rootstock / Kor. J. Hort. Sci. Technol. 22 : 36 ~
Capone I / 1989 / Induction and growth properties of carrot roots with different complements of Agrobacterium rhizogenes T-DNA / Plant Mol Biol. 13 (1) : 43 ~ 52
Fladung M / 1990 / Transformation of diploid and tetraploid potato clones with the rolC gene of Agrobacterium rhizogenes and characterization of transgenic plants / Plant Bre
Gardner N / 2006 / Differential expression of rolC results in unique plant phenotypes / J. Amer. Soc. Hort. Sci. 131 (1) : 82 ~ 88
Han SG / 2004 / Figure of freezing injury in apple trees on M.9 rootstock / Kor. J. Hort. Sci. Technol. 22 : 36 ~
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