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논문 기본 정보
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- 저자정보
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- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제16권 제2호
- 발행연도
- 2008.1
- 수록면
- 87 - 92 (6page)
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Bombyx mori nucleopolyhedrovirus (BmNPV) orf47 gene was characterized for the first time. The coding sequence of Bm47 was amplified and subcloned into the prokaryotic expression vector pET-30a(+) in order to produce His-tagged fusion protein in the BL21 (DE3) cells. The His-Bm47 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. As the genome of BmNPV is available in GenBank and the EST database of BmNPV is expanding, identification of novel genes of BmNPV was conceivable by data-mining techniques and bioinformatics tools. Structural bioinformatics approach to analyze the properties of Bm47 encodes protein.
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참고문헌 (18)
참고문헌 신청
Acharya, A. / 2002 / “Transcriptional analysis and preliminary characterization of ORF Bm42 from Bombyx mori nucleopolyhedrovirus.” / Virology 299 (2) : 213 ~ 224
Ayres, M. D. / 1994 / “The complete DNA sequence of Autographa californica nuclear polyhedrosis virus.” / Virology 202 (2) : 586 ~ 605
Baluchamy, S. / 2005 / “Characterization of a cyclin homolog from Bombyx mori nucleopolyhedrovirus.” / Virus Res 108 (1-2) : 69 ~ 81
Chen, H. Q. / 2007 / “Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus.” / FEBS Lett 581 (30) : 5836 ~ 5842
Daimon, T. / 2007 / “Mutational analysis of active site residues of chitinase from Bombyx mori nucleopolyhedrovirus.” / Virus Res 124 (1-2) : 168 ~ 175
Ayres, M. D. / 1994 / “The complete DNA sequence of Autographa californica nuclear polyhedrosis virus.” / Virology 202 (2) : 586 ~ 605
Baluchamy, S. / 2005 / “Characterization of a cyclin homolog from Bombyx mori nucleopolyhedrovirus.” / Virus Res 108 (1-2) : 69 ~ 81
Chen, H. Q. / 2007 / “Characterization of a late gene, ORF67 from Bombyx mori nucleopolyhedrovirus.” / FEBS Lett 581 (30) : 5836 ~ 5842
Daimon, T. / 2007 / “Mutational analysis of active site residues of chitinase from Bombyx mori nucleopolyhedrovirus.” / Virus Res 124 (1-2) : 168 ~ 175
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