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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제18권 제1호
- 발행연도
- 2009.1
- 수록면
- 33 - 40 (8page)
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초록· 키워드
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A vitellogenin cDNA was cloned from the bumblebee Bombus ignitus. The cDNA encoding B. ignitus vitellogenin (BiVg) is 5473 bases long with an open reading frame of 1773 amino acid residues. BiVg possesses two consensus (RXXR/S) cleavage sites and has the conserved DGXR and GL/ICG motif near its C-terminus.
The deduced amino acid sequence of BiVg cDNA showed significant similarity with hymenopteran Vgs (51% identity to Apis mellifera Vg, and 33~36% to other insect Vgs). The BiVg cDNA was expressed as a 200-kDa polypeptide in baculovirus-infected insect Sf9 cells. Northern and Western blot analyses showed the expression of BiVg in fat bodies of pupae and adults.
In queens, the expression of BiVg was first detected in late pupal stage fat bodies, and secreted BiVg was also observed in late pupal stage hemolymph.
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참고문헌 (23)
참고문헌 신청
[학술지(정
Barchuk AR. / 2002 / Effects of juvenile hormone and ecdysone on the timing of vitellogenin appearance in hemolymph of queen and worker pupae of Apis mellifera / J Insect Sci
Bownes M. / 1986 / Expression of gene coding for vitellogenin (yolk protein) / Annu Rev Entomol 31 : 507 ~ 531
Byrne BM. / 1989 / The evolution of egg yolk proteins / Prog Biophys Mol Biol. 53 : 33 ~ 69
Chen JS. / 1994 / Analysis of mosquito vitellogenin cDNA similarity with vertebrate phosvitins and arthropod serum proteins / J Mol Biol. 237 : 641 ~ 647
Barchuk AR. / 2002 / Effects of juvenile hormone and ecdysone on the timing of vitellogenin appearance in hemolymph of queen and worker pupae of Apis mellifera / J Insect Sci
Bownes M. / 1986 / Expression of gene coding for vitellogenin (yolk protein) / Annu Rev Entomol 31 : 507 ~ 531
Byrne BM. / 1989 / The evolution of egg yolk proteins / Prog Biophys Mol Biol. 53 : 33 ~ 69
Chen JS. / 1994 / Analysis of mosquito vitellogenin cDNA similarity with vertebrate phosvitins and arthropod serum proteins / J Mol Biol. 237 : 641 ~ 647
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