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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 1 - 7 (7page)
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Background: In the present study, various freezing containers were tested for mouse embryos of respective developmental stages; embryos were vitrified and then their survival rate and developmental rate were monitored.
Mouse two cell, 8 cell, and blastula stage embryos underwent vitrification freezing-thawing and then their recovery rate, survival rate, development rate, and hatching rate were investigated.
Methods: EM-grid, OPS, and cryo-loop were utilized for vitrification freezing-thawing of mouse embryos.
Results: It was found that recovery rate and survival rate were higher in the group of cryo-loop compared to those of EM-grid (p < 0.05). Embryonic development rate, two cell embryos to blastocyst, as well as hatching rate were higher in the control group compared to the EM-grid group and OPS group (p < 0.05), yet no difference was noted between the control group and cryo-loop group. Development rate and hatching rate of eight cell morulae and blastocysts were all lower in the treatment groups than the control group whilst hatching rate of blastocysts was higher in the control group compared to the groups of EM-grid and OPS (p < 0.05); although the cryo-loop group was shown to be slightly higher than other groups, it was not statistically significant.
Conclusions: In the study, we investigate effects of freezing containers on vitrified embryos of respective developmental stages; it was demonstrated that higher developmental rate was shown in more progressed (or developed) embryos with more blastomeres. There was however, no difference in embryonic development rate was shown amongst containers. Taken together, further additional studies are warranted with regards to 1) manipulation techniques of embryos for various vitrification freezing containers and 2) preventive measures against contamination via liquid nitrogen.
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참고문헌 (32)
참고문헌 신청
Bautista S / 1998 / In breast cancer, amplification of the steroid receptor coactivator gene AIB1 is correlated with estrogen and progesterone receptor positivity / Clin Cancer Res 4(12):2925~2929
Bolton VN / 1989 / Development of spare implantation embryos in vitro: an analysis of the correlations among gross morphology, cleavage rates and development to the blastocyst / J In Vitro Fert Embryo Transf 6:30~35
Chen SU / 2000 / Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws / Hum Reprod 15:2598~2603
Cho HJ / 2002 / An improved protocol for dilution of cryoprotectant from vitrified human blastocyst / Hum Reprod 17(2419?2):22
Choi DH / 2000 / Pregnancy and delivery of healthy infants developed from vitrified blastocysts in an IVF-ET program / Fertil Steril 74:838~839
Bolton VN / 1989 / Development of spare implantation embryos in vitro: an analysis of the correlations among gross morphology, cleavage rates and development to the blastocyst / J In Vitro Fert Embryo Transf 6:30~35
Chen SU / 2000 / Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws / Hum Reprod 15:2598~2603
Cho HJ / 2002 / An improved protocol for dilution of cryoprotectant from vitrified human blastocyst / Hum Reprod 17(2419?2):22
Choi DH / 2000 / Pregnancy and delivery of healthy infants developed from vitrified blastocysts in an IVF-ET program / Fertil Steril 74:838~839
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