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- To study the radiation related gene expression in mutants of Bacilluslentimorbus WJ5 induced by gamm radiation, the simultaneous gene expression wasanalyzed by DNA microarray. We constructed DNA chips including two thousandrandomly digested genome spots of B. lentimorbus WJ5 and compared its quantitativeaspect with seven mutants induced by gamma radiation (60Co). From the clusteranalysis of gene expression pattern, totally 408 genes were expressed and 27 genesgenes involved in repair( mutL, mutM ), energy metabolism( acsA, sdhB, pgk, yhjB , citB),protease ( npr ), and reduction response to oxidative stress (HMM) were simultaneouslyupregulated. It seems that the induction of the direct and/or indirect repair relatedgenes in mutants induced by gamma radiation could be remarkably different from theadaptive responses against acute exposure to radiation.
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참고문헌 (22)
참고문헌 신청
Becker D / 1993 / The chemical consequencesof radiation damage to DNA : 121 ~ 180
Bernadette BN / 1997 / Induction of stressproteins in the phototrophic bacterium Rhodobactersphaeroides 153 : 173 ~ 180
Chang H-H / 2003 / Mutation spectrum of manganese peroxidasegene in Pleurotus ostreatus mutants induced by gammaradiation 41 : 52 ~ 57
Chen YJ / 2003 / Normalization methods for analysis ofmicroarray gene-expression data 13: : 57 ~ 74
Colantuoni C, / 2002b / Local mean normalization of microarray element signalintensities across an array surface quality control andcorrection of spatially systematic artifac
Bernadette BN / 1997 / Induction of stressproteins in the phototrophic bacterium Rhodobactersphaeroides 153 : 173 ~ 180
Chang H-H / 2003 / Mutation spectrum of manganese peroxidasegene in Pleurotus ostreatus mutants induced by gammaradiation 41 : 52 ~ 57
Chen YJ / 2003 / Normalization methods for analysis ofmicroarray gene-expression data 13: : 57 ~ 74
Colantuoni C, / 2002b / Local mean normalization of microarray element signalintensities across an array surface quality control andcorrection of spatially systematic artifac
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