메뉴 건너뛰기
.. 내서재 .. 알림
소속 기관/학교 인증
인증하면 논문, 학술자료 등을  무료로 열람할 수 있어요.
한국대학교, 누리자동차, 시립도서관 등 나의 기관을 확인해보세요
(국내 대학 90% 이상 구독 중)
로그인 회원가입 고객센터 ENG
주제분류
인문학
인문학 일반 역사학 철학 종교학 어문학 기타 인문학
사회과학
사회과학 일반 경영학 관광학 교육학 군사학 문헌정보학 법학 사회학 신문방송학 심리과학 정치외교학 지리학 행정학 경제학 무역학 인류학 기타 사회과학
자연과학
자연과학 일반 물리학 생물학 생활과학 수학·통계학 천문학 지구과학 화학 기타 자연과학
공학
공학 일반 건축공학 기계공학 산업공학 재료·에너지공학 전기전자공학 조선해양공학 화학공학 컴퓨터학 기타공학
의약학
의학 일반 간호학 수의학 약학 한의학 기초의학 임상의학 기타 의약학
농수해양학
농수해양학 일반 농학 수산학 식품과학 임학 축산학 해상운송학 기타 농수해양학
예술체육학
예술 일반 디자인 미술 미용 사진 음악학 의상 체육 공연매체예술 기타 예술체육학
복합학
학제간연구 과학기술학 뇌인지과학 기술정책 기타 복합학

ENG

추천
검색

논문 기본 정보

자료유형
학술저널
저자정보
저널정보
한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제1호
발행연도
2017.1
수록면
179 - 188 (10page)

이용수

표지
📌
연구주제
📖
연구배경
🔬
연구방법
🏆
연구결과
AI에게 요청하기
추천
검색

초록· 키워드

오류제보하기
White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the 7th day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.
#Lignin #ligninolytic enzymes #white-rot basidiomycetes

목차

등록된 정보가 없습니다.

참고문헌 (1)

참고문헌 신청
Chen M / 2011 / Understanding lignin-degrading reactions of ligninolytic enzymes: binding affinity and interactional profile / PLoS One 6 : 25647 ~ | 2. [학술지(정기간행물)] - Kües U. / 2015 / Fungal enzymes for environmental management / Curr. Opin. Biotechnol 33 : 268 ~ 278 | 3. [학술지(정기간행물)] - Silva R / 2009 / Aplicações de fibras lignocelulósicas na Química de polímeros e em compósitos / Química Nova 32 : 661 ~ 671 | 4. [학술지(정기간행물)] - Singh R / 2014 / A review on delignification of lignocellulosic biomass for enhancement of ethanol production potential / Renew. Sustain. Energy Rev 32 : 713 ~ 728 | 5. [학술지(정기간행물)] - Lundell TK / 2010 / Lignin-modifying enzymes in filamentous basidiomycetes – ecological, functional and phylogenetic review / J. Basic Microbiol 50 : 5 ~ 20 | 6. [단행본] - Durán N / 2010 / FUNGOS uma Introdução a Biologia, Bioquímica e Biotecnologia / EDUCS | 7. [단행본] - Polizelli MLTM / 2014 / Fungal Enzymes / CRC Press | 8. [학술지(정기간행물)] - Quiroz-Castañeda RE / 2011 / Evaluation of different lignocellulosic substrates for the production of cellulases and xylanases by the basidiomycete fungi Bjerkandera adusta and Pycnoporus sanguineus / Biodegradation 22 : 565 ~ 572 | 9. [학술지(정기간행물)] - Wong DWS. / 2009 / Structure and action mechanism of ligninolytic enzymes / Appl. Microbiol. Biotechnol 157 : 174 ~ 209 | 10. [학술지(정기간행물)] - Cantarel BL / 2009 / The Carbohydrate-Active EnZymes database (CAZy): an expert resource for glycogenomics / Nucleic Acids Res 37 : 233 ~ 238 | 11. [학술지(정기간행물)] - Zhao Z / 2014 / Comparative analysis of fungal genomes reveals different plant cell wall degrading capacity in fungi / BMC Genomics 15 : 6 ~ | 12. [학술지(정기간행물)] - Ospina-Giraldo MD / 2010 / The CAZyome of Phytophthora spp: a comprehensive analysis of the gene complement coding for carbohydrateactive enzymes in species of the genus Phytophthora / BMC Genomics 11 : 525 ~ | 13. [학술지(정기간행물)] - Matheny PB / 2007 / Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi) / Mol. Phylogenet. Evol 43 : 430 ~ 451 | 14. [학술지(정기간행물)] - Riley R / 2014 / Extensive sampling of basidiomycete genomes demonstrates inadequacy of the white-rot/brownrot paradigm for wood decay fungi / Proc. Natl. Acad. Sci. USA 111 : 9923 ~ 9928 | 15. [학술지(정기간행물)] - Xu J / 2007 / Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens / Proc. Natl. Acad. Sci. USA 104 : 18730 ~ 18735 | 16. [학술지(정기간행물)] - Brown NA / 2012 / The predicted secretome of the plant pathogenic fungus Fusarium graminearum: a refined comparative analysis / PLoS One 7 : e33731 ~ | 17. [학술지(정기간행물)] - Levasseur A / 2013 / Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes / Biotechnol. Biofuels 6 : 41 ~ | 18. [학술지(정기간행물)] - Seshime Y / 2010 / Identification of csypyrone B1 as the novel product of Aspergillus oryzae type III polyketide synthase CsyB / Bioorg. Med. Chem 18 : 4542 ~ 4546 | 19. [학술지(정기간행물)] - Matheny PB / 2006 / Resolving the phylogenetic position of the Wallemiomycetes: an enigmatic major lineage of Basidiomycota / Can. J. Bot 84 : 1794 ~ 1805 | 20. [학술지(정기간행물)] - Hori C / 2013 / Genomewide analysis of polysaccharides degrading enzymes in 11 white- and brownrot Polyporales provides insight into mechanisms of wood decay / Mycologia 105 : 1412 ~ 1427 | 21. [학술지(정기간행물)] - Kirk TK / 1987 / Enzymatic “combustion”: the microbial degradation of lignin / Annu. Rev. Microbiol 41 : 465 ~ 505 | 22. [학술지(정기간행물)] - Wariishi H / 1992 / Manganese(II)oxidation by manganese peroxidase from the basidiomycete Phanerochaete chrysosporium. Kinetic mechanism and role of chelators / J. Biol. Chem 267 : 23688 ~ 23695 | 23. [학술지(정기간행물)] - Moredo N / 2003 / Enhanced ligninolytic enzyme production and degrading capability of Phanerochaete chrysosporium and Trametes versicolor / World J. Microbiol. Biotechnol 19 : 665 ~ 669 | 24. [학술지(정기간행물)] - Singh AP / 2014 / Biotechnological applications of wood-rotting fungi: a review / Biomass Bioenergy 62 : 198 ~ 206 | 25. [학술지(정기간행물)] - Wang P / 2008 / Effect of culture conditions on the production of ligninolytic enzymes of white-rot fungi Phanerochaete chrysosporium (ATCC 20696) and separation of its lignin peroxidase / World J. Microbiol. Biotechnol 24 : 2205 ~ 2212 | 26. [학술지(정기간행물)] - Dashtban M / 2010 / Fungal biodegradation and enzymatic modification of lignin / Int. J. Biochem. Mol. Biol 1 : 36 ~ 50 | 27. [단행본] - Ferraz AL / 2010 / FUNGOS uma Introdução a Biologia, Bioquímica e Biotecnologia / EDUCS | 28. [학술지(정기간행물)] - Hammel K / 2008 / Role of fungal peroxidases in biological ligninolysis / Curr. Opin. Biotechnol 11 : 349 ~ 355 | 29. [학술지(정기간행물)] - Ruiz-Dueñas F / 2009 / Substrate oxidation sites in versatile peroxidase and other basidiomycete peroxidases / J. Exp. Bot 60 : 441 ~ 452 | 30. [학술지(정기간행물)] - Morgenstern I / 2008 / Molecular evaluation and diversity of lignin degrading heme peroxidase in the Agaricomycetes / J. Mol. Evol 66 : 243 ~ 257 | 31. [단행본] - Neves MA / 2013 / Guide to the Common Fungi of the Semiarid Region of Brazil / Tecc Editora Ltda | 32. [학술지(정기간행물)] - Bukh C / 2006 / Kinetic studies on the reaction between Trametes villosa laccase and dioxygen / J. Inorg. Biochem 100 : 1547 ~ 1557 | 33. [학술지(정기간행물)] - Morozova OV / 2007 / Laccase-mediator system and their applications: a review / Appl. Biochem. Microbiol 43 : 523 ~ 535 | 34. [학술지(정기간행물)] - Tadesse MA / 2008 / An assessment of the relative contributions of redox and steric issues to laccase specificity towards putative substrates / Org. Biomol. Chem 6 : 868 ~ 878 | 35. [학술지(정기간행물)] - Silva MLC / 2014 / Production of manganese peroxidase by Trametes villosa on inexpensive substrate and its application in the removal of lignin from agricultural wastes / Adv. Biosci. Biotechnol 5 : 1067 ~ 1077 | 36. [학술지(정기간행물)] - Yamanaka R / 2008 / Lignolytic enzymes produced by Trametes villosa CCB176under different culture conditions / Braz. J. Microbiol 39 : 78 ~ 84 | 37. [학술지(정기간행물)] - Yaver DS / 1996 / Purification, characterization, molecular cloning, and expression of two laccase genes from the white-rot basidiomycete Trametes villosa / Appl. Environ. Microbiol 62 : 834 ~ 841 | 38. [학술지(정기간행물)] - Kuwahara M / 1984 / Separation and characterization of two extracellular H2O2dependent oxidases from lignolytic cultures of Phanerochaete chrysosporium / FEBS Lett 169 : 247 ~ 250 | 39. [학술지(정기간행물)] - Tien M / 1984 / Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase / Proc. Natl. Acad. Sci. USA 81 : 2280 ~ 2284 | 40. [학술지(정기간행물)] - Doyle JJ / 1987 / A rapid DNA isolation procedure for small quantities of fresh leaf tissue / Phytochem. Bull 19 : 11 ~ 15 | 41. [학술지(정기간행물)] - Góes-Neto A / 2005 / DNA extraction from frozen field-collected and dehydrated herbarium fungal basidiomata: performance of SDS and CTAB-based methods / Biotemas 18 : 19 ~ 32 | 42. [학술지(정기간행물)] - Livak KJ / 2001 / Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method / Methods 25 : 402 ~ 408 | 43. [학술지(정기간행물)] - Fitch WM / 1967 / Construction of phylogenetic trees / Science 155 : 279 ~ 284 | 44. [학술지(정기간행물)] - Janusz G / 2013 / Fungal laccase, manganese peroxidase and lignin peroxidase: gene expression and regulation / Enzyme Microb. Technol 52 : 1 ~ 12 | 45. [학술지(정기간행물)] - Galhaup C / 2002 / Characterization of the major laccase isoenzyme from Trametes pubescens and regulation of its synthesis by metal ions / Microbiology 148 : 2159 ~ 2169 | 46. [학술지(정기간행물)] - Ferhan M / 2013 / Identification of a potential fungal species by 18S rDNA for ligninases production / World J. Microbiol. Biotechnol 29 : 2437 ~ 2440 | 47. [학술지(정기간행물)] - Iqbal HMN / 2011 / Optimization of physical and nutritional factors for synthesis of lignin degrading enzymes by a novel strain of Trametes versicolor / Bioresources 6 : 1273 ~ 1278 | 48. [학술지(정기간행물)] - Guerra G / 2008 / Production of laccase and manganese peroxidase by the white-rot fungi from sugarcane bagasse in solid bed use for dyes decolourisation / Sugar Technol 10 : 260 ~ 264 | 49. [단행본] - Galvagno MA / 2010 / FUNGOS uma Introdução a Biologia, Bioquímica e Biotecnologia / EDUCS | 50. [학술지(정기간행물)] - Rakrudee Sarnthima / 2009 / Extracellular Ligninolytic Enzymes by Lentinus polychrous Lév. under Solid-state Fermentation of Potential Agro-industrial Wastes and Their Effectiveness in Decolorization of Synthetic Dyes / Biotechnology and Bioprocess Engineering 14 (4) : 513 ~ 522 | 51. [학술지(정기간행물)] - Levin L / 2010 / Effect of nitrogen sources and vitamins on ligninolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates / Bioresour. Technol 101 : 4554 ~ 4563 | 52. [학술지(정기간행물)] - Johasson T / 1996 / A cluster of genes encoding major isozymes of lignin peroxidase, and manganese peroxidase from the white-rot fungus Trametes versicolor / Gene 170 : 31 ~ 38 | 53. [학술지(정기간행물)] - Cullen D. / 1997 / Recent advances on the molecular genetics of ligninolytic fungi / J. Biotechnol 53 : 273 ~ 289 | 54. [학술지(정기간행물)] - Pérez-Boada M / 2005 / Versatile peroxidase oxidation of high redox potential aromatic compounds: sitedirected mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways / J. Mol. Biol 354 : 385 ~ 402 | 55. [학술지(정기간행물)] - Floudas D / 2012 / The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes / Science 336 : 1715 ~ 1719 | 56. [학술지(정기간행물)] - Knežević A / 2013 / Potential of Trametes species to degrade lignin / Int. Biodeterior. Biodegradation 85 : 52 ~ 56 | 57. [학술지(정기간행물)] - Dinis MJ / 2009 / Modification of wheat straw lignin by solid state fermentation with white-rot fungi / Bioresour. Technol 100 : 4829 ~ 4835 | 58. [학술지(정기간행물)] - Elisashvili V / 2008 / Effect of growth substrate, method of fermentation, and nitrogen source on lignocellulose-degrading enzymes production by white-rot basidiomycetes / J. Ind. Microbiol. Biotechnol 35 : 1531 ~ 1538 | 59. [학술지(정기간행물)] - Koyani RD / 2015 / Solid state fermentation:comprehensive tool for utilization of lignocellulosic through biotechnology / J. Bioprocess Biotech 5 : 2 ~ google schola

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!

이 논문의 저자 정보

최근 본 자료

전체보기

댓글(0)

0