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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제6호
- 발행연도
- 2015.1
- 수록면
- 803 - 813 (11page)
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초록· 키워드
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The growth of Irpex lacteus F17 and manganese peroxidase (MnP) production in a selfdesigned tray bioreactor, operating in solid-state conditions at a laboratory scale, were studied. The bioreactor was divided into three layers by three perforated trays. Agroindustrial residues were used both as the carrier of bound mycelia and as a nutrient medium for the growth of I. lacteus F17. The maximum biomass production in the bioreactor was detected at 60 h of fermentation, which was consistent with the CO2 releasing rate by the fungus. During the stationary phase of fungal growth, the maximum MnP activity was observed, reaching 950 U/l at 84 h. Scanning electron microscopy images clearly showed the growth situation of mycelia on the support matrix. Furthermore, the MnP produced by I. lacteus F17 in the bioreactor was isolated and purified, and the internal peptide sequences were also identified with mass spectrometry. The optimal activity of the enzyme was detected at pH 7 and 25°C, with a long half-life time of 9 days. In addition, the MnP exhibited significant stability within a broad pH range of 4-7 and at temperature up to 55°C. Besides this, the MnP showed the ability to decolorize the polymeric model dye Poly R-478 in vitro.
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참고문헌 (23)
참고문헌 신청
Aslam S / 2011 / Partial purification and characterization of ligninolytic enzymes produced by Pleurotus ostreatus during solid state fermentation / Afr. J. Biotechnol 10 : 1
Baborova P / 2006 / Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus, and degradation of polycyclic aromatic hydrocarbons by the enzyme / Res.
Boer CG / 2006 / Purification and some properties of Mn peroxidase from Lentinula edodes / Process Biochem 41 : 1203 ~ 1207
Couto SR / 2003 / Investigation of several bioreactor configurations for laccase production by Trametes versicolor operating in solid-state conditions / Biochem. Eng. J 15 :
Couto SR / 2005 / Application of solid-state fermentation to ligninolytic enzyme production / Biochem. Eng. J 22 : 211 ~ 219
Baborova P / 2006 / Purification of a new manganese peroxidase of the white-rot fungus Irpex lacteus, and degradation of polycyclic aromatic hydrocarbons by the enzyme / Res.
Boer CG / 2006 / Purification and some properties of Mn peroxidase from Lentinula edodes / Process Biochem 41 : 1203 ~ 1207
Couto SR / 2003 / Investigation of several bioreactor configurations for laccase production by Trametes versicolor operating in solid-state conditions / Biochem. Eng. J 15 :
Couto SR / 2005 / Application of solid-state fermentation to ligninolytic enzyme production / Biochem. Eng. J 22 : 211 ~ 219
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