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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Single-chain antibody variable fragment (scFv) is widely used at screening stage. However, the scFv format often proves tobe inefficient in downstream stages such as crystallization and in vivo applications. Conversion of a scFv format to otherones such as a fragment antigen binding (Fab), a scFv-fragment crystallizable region (scFv-Fc), a scFv-constant heavychain (scFv-CH) and an immunoglobulin (IgG) formats can be cumbersome due to different sets of restriction enzyme sitesin multiple vectors. To facilitate antibody format conversion in laboratory settings, we developed a cloning platform, called“AbVec”, with the common restriction enzyme sites across various forms of vectors. In the AbVec platform, a scFv cloneselected from phage display is modularized into VH and VL regions and transferred to vectors for Fab, scFv-Fc, scFv-CH and IgG production using the same restriction enzyme sites. Using scFv F9, specifically recognizing a pneumococcalsecreted peptide, we demonstrated the AbVec platform yielded the expression vectors within a week. Different formats ofthe antibodies were successfully expressed and purified homogenously, supporting the usefulness of the AbVec platform. The AbVec platform will facilitate otherwise time-consuming conversion process in laboratory settings.
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참고문헌 (17)
참고문헌 신청
Barbas, C. F. / 2001 / Phage display: a laboratory manual / Cold Spring Harbor Laboratory Press
Beck, A. / 2017 / Strategies and challenges for the next generation of antibody–drug conjugates / Nat Rev Drug Discov 16:315
Bujak, E. / 2014 / Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification / Methods Mol Biol 1131:315~334
Emmons, C. / 1987 / Muromonab-CD3(Orthoclone OKT3) : the first monoclonal antibody approved for therapeutic use / Iowa Med 77:78
Jones, M. L. / 2010 / A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies / J Immunol Methods 354:85~90
Beck, A. / 2017 / Strategies and challenges for the next generation of antibody–drug conjugates / Nat Rev Drug Discov 16:315
Bujak, E. / 2014 / Reformatting of scFv antibodies into the scFv-Fc format and their downstream purification / Methods Mol Biol 1131:315~334
Emmons, C. / 1987 / Muromonab-CD3(Orthoclone OKT3) : the first monoclonal antibody approved for therapeutic use / Iowa Med 77:78
Jones, M. L. / 2010 / A method for rapid, ligation-independent reformatting of recombinant monoclonal antibodies / J Immunol Methods 354:85~90
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