지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.9
- 수록면
- 773 - 780 (8page)
- DOI
- 10.14348/molcells.2015.0073
이용수
초록· 키워드
오류제보하기
3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV- 1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV.
PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and 10 μg 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 LD50 PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% (5 μg) and 47% (10 μg).
The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.
목차
등록된 정보가 없습니다.
참고문헌 (39)
참고문헌 신청
Akaike, T. / 1996 / Pathogenesis of influenza virus-induced pneumonia : involvement of both nitric oxide and oxygen radicals / Proc. Natl. Acad. Sci. USA 93:2448~2453
Banks, P. R. / 1995 / Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis / Bioconjug. Chem 6:447~458
Boado, R. J. / 2010 / Pharmacokinetics and brain uptake of a genetically engineered bifunctional fusion antibody targeting the mouse transferrin receptor / Mol. Pharm 7:237~244
Breshears, M. A. / 2005 / Temporal progression of viral replication and gross and histological lesions in Balb/c mice inoculated epidermally with Saimiriine herpesvirus 1(SaHV-1) / J. Comp. Pathol 133:103~113
Brinkley, M. / 1992 / A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents / Bioconjug. Chem 3:2~13
Banks, P. R. / 1995 / Comparison of three common amine reactive fluorescent probes used for conjugation to biomolecules by capillary zone electrophoresis / Bioconjug. Chem 6:447~458
Boado, R. J. / 2010 / Pharmacokinetics and brain uptake of a genetically engineered bifunctional fusion antibody targeting the mouse transferrin receptor / Mol. Pharm 7:237~244
Breshears, M. A. / 2005 / Temporal progression of viral replication and gross and histological lesions in Balb/c mice inoculated epidermally with Saimiriine herpesvirus 1(SaHV-1) / J. Comp. Pathol 133:103~113
Brinkley, M. / 1992 / A brief survey of methods for preparing protein conjugates with dyes, haptens, and cross-linking reagents / Bioconjug. Chem 3:2~13
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