지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 460 - 469 (10page)
이용수
초록· 키워드
오류제보하기
Bacterial α-type carbonic anhydrase (α-CA) is a zinc metalloenzyme that catalyzes the reversible and extremely rapid interconversion of carbon dioxide to bicarbonate. In this study, we report the first crystal structure of a hyperthermostable α-CA from Persephonella marina EX-H1 (pmCA) in the absence and presence of competitive inhibitor, acetazolamide. The structure reveals a compactly folded pmCA homodimer in which each monomer consists of a 10-stranded β-sheet in the center. The catalytic zinc ion is coordinated by three highly conserved histidine residues with an exchangeable fourth ligand (a water molecule, a bicarbonate anion, or the sulfonamide group of acetazolamide). Together with an intramolecular disulfide bond, extensive interfacial networks of hydrogen bonds, ionic and hydrophobic interactions stabilize the dimeric structure and are likely responsible for the high thermal stability. We also identified novel binding sites for calcium ions at the crystallographic interface, which serve as molecular glue linking negatively charged and otherwise repulsive surfaces. Furthermore, this large negatively charged patch appears to further increase the thermostability at alkaline pH range via favorable charge-charge interactions between pmCA and solvent molecules. These findings may assist development of novel α-CAs with improved thermal and/or alkaline stability for applications such as CO2 capture and sequestration.
목차
등록된 정보가 없습니다.
참고문헌 (61)
참고문헌 신청
Alber, BE. / 1994 / A carbonic anhydrase from the archaeon Methanosarcina thermophila / Proc. Natl. Acad. Sci. U. S. A 91:6909~6913
Alterio, V. / 2015 / Cadmiumcontaining carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii / Mar. Drugs 13:1688~1697
Boone, C. D. / 2013 / Structural and catalytic characterization of a thermally stable and acid-stable variant of human carbonic anhydrase II containing an engineered disulfide bond / Acta Crystallogr. D Biol. Crystallogr 69:1414~1422
Britton, K. L. / 2006 / Analysis of protein solvent interactions in glucose dehydrogenase from the extreme halophile Haloferax mediterranei / Proc. Natl. Acad. Sci. U. S. A 103:4846~4851
Carter, J. M. / 1969 / Electrometric assay of rate of hydration of CO2 for investigation of kinetics of carbonic anhydrase / J. Physiol 204:60P~62P
Alterio, V. / 2015 / Cadmiumcontaining carbonic anhydrase CDCA1 in marine diatom Thalassiosira weissflogii / Mar. Drugs 13:1688~1697
Boone, C. D. / 2013 / Structural and catalytic characterization of a thermally stable and acid-stable variant of human carbonic anhydrase II containing an engineered disulfide bond / Acta Crystallogr. D Biol. Crystallogr 69:1414~1422
Britton, K. L. / 2006 / Analysis of protein solvent interactions in glucose dehydrogenase from the extreme halophile Haloferax mediterranei / Proc. Natl. Acad. Sci. U. S. A 103:4846~4851
Carter, J. M. / 1969 / Electrometric assay of rate of hydration of CO2 for investigation of kinetics of carbonic anhydrase / J. Physiol 204:60P~62P
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Crystal Structure of β-Carbonic Anhydrase CafA from the Fungal Pathogen Aspergillus fumigatus
Molecules and Cells
2020 .01
-
Structural analysis of the non-catalytic zinc binding site in the minor β-carbonic anhydrase CafD
Biodesign
2019 .01
-
CO₂ Mineralization by the Recombinant Strains Expressing Thermostable Carbonic Anhydrases
한국미생물학회 학술대회논문집
2015 .04
-
Stabilization of Microbial Carbonic Anhydrase for CO₂ Conversion and Utilization
한국미생물학회 학술대회논문집
2021 .08
-
An Extremely Thermostable and Halophilic β-Class Carbonic Anhydrase from Thermovibrio ammonificans as a Biocatalyst for CO₂ Capture
한국미생물학회 학술대회논문집
2022 .10
-
Molecular Docking Studies of Wolbachia Endosymbiont of Brugia Malayi's Carbonic Anhydrase Using Coumarin-chromene Derivatives Towards Designing Anti-filarial Agents
조선자연과학논문집
2016 .01
-
Charges of TIP4P water model for mixed quantum/classical calculations of OH stretching frequency in liquid water
Rapid communication in photoscience : RCP
2016 .01
-
토양 중 탄산무수화효소 보유 박테리아를 위한 프라이머 개발
대한지질학회 학술대회
2020 .10
-
Adsorption of Uniformly Charged Soft and Hard Spherical Nanoparticles
새물리
2023 .03
-
Study of Driving and Thermal Stability of Anode-type Ion Beam Source by Charge Repulsion Mechanism
Applied Science and Convergence Technology
2018 .05
-
Coordination of an Amino Alcohol Schiff Base Ligand Toward Cd(Ⅱ)
대한화학회지
2019 .02
-
Statistical Characterization of the Multi-Charged Fragment Ions in the CID and HCD Spectrum
Mass Spectrometry Letters
2021 .06
-
Synthesis and Characterization of Zinc Ion Imprinted Polymer for Selective Adsorption of Zinc Ion and Its Application
한국분석과학회 학술대회
2019 .11
-
Synthesis and Characterization of Zinc Ion Imprinted Polymer for Selective Adsorption of Zinc Ion and Its Application
한국분석과학회 학술대회
2019 .11
-
Structural Analyses of Zinc Finger Domains for Specific Interactions with DNA
Journal of Microbiology and Biotechnology
2016 .12
-
Mass Spectrometric Determination of Zn2+ Binding/Dissociation Constant for Zinc Finger Peptides
Mass Spectrometry Letters
2015 .03
-
Stability and Electronic Properties of the Adsorption of Molecular Hydrogen on Metal-containing Single-walled Carbon Nanotubes
대한화학회지
2015 .10
-
Relationship between intermolecular packing arrangement and charge transports in organic single crystals
한국진공학회 학술발표회초록집
2020 .02
-
Role of hydrogen bond network in the function, stability, and structure of ketosteroid isomerase from Pseudomonas putida
Biodesign
2015 .06
-
Investigating InSnZnO as an active layer for non-volatile memory devices and increasing memory window by utilizing Silicon-rich SiOx for charge storage layer
한국진공학회 학술발표회초록집
2016 .08
이 논문의 저자 정보
최근 본 자료
댓글(0)
0