지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제3호
- 발행연도
- 2019.1
- 수록면
- 454 - 464 (11page)
이용수
초록· 키워드
오류제보하기
Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, MgSO4 concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non- Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as 89 fg/μl, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.
목차
등록된 정보가 없습니다.
참고문헌 (48)
참고문헌 신청
Tang F / 2016 / Visual and efficient immunosensor technique for advancing biomedical applications of quantum dots on Salmonella detection and isolation / Nanoscale 8:4688~4698
Lahiri C / 2014 / Interactome analyses of Salmonella pathogenicity islands reveal SicA indispensable for virulence / J. Theor. Biol 363:188~197
Vohra P / 2017 / Quantifying the survival of multiple Salmonella enterica serovars in vivo using massively-parallel whole genome sequencing to predict zoonotic risk / Appl. Environ. Microbiol 84:e02262-17
Ji Y / 2017 / QseB mediates biofilm formation and invasion in Salmonella enterica serovar Typhi / Microb. Pathog 104:6~11
Patterson PH / 2014 / Introduction : reducing Salmonella Enteritidis contamination of shell eggs / J. Appl. Poultry Res 23:323~329
Lahiri C / 2014 / Interactome analyses of Salmonella pathogenicity islands reveal SicA indispensable for virulence / J. Theor. Biol 363:188~197
Vohra P / 2017 / Quantifying the survival of multiple Salmonella enterica serovars in vivo using massively-parallel whole genome sequencing to predict zoonotic risk / Appl. Environ. Microbiol 84:e02262-17
Ji Y / 2017 / QseB mediates biofilm formation and invasion in Salmonella enterica serovar Typhi / Microb. Pathog 104:6~11
Patterson PH / 2014 / Introduction : reducing Salmonella Enteritidis contamination of shell eggs / J. Appl. Poultry Res 23:323~329
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
LAMP-3 (Lysosome-Associated Membrane Protein 3) Promotes the Intracellular Proliferation of Salmonella typhimurium
Molecules and Cells
2016 .07
-
Rapid and Visual Detection of Vibrio parahaemolyticus in Aquatic Foods Using blaCARB-17 Gene-Based Loop-Mediated Isothermal Amplification with Lateral Flow Dipstick (LAMP-LFD)
Journal of Microbiology and Biotechnology
2021 .12
-
Establishment and Application of Polymerase Spiral Reaction Amplification for Salmonella Detection in Food
Journal of Microbiology and Biotechnology
2019 .01
-
Cell Mediated Immune Responses Induced by Salmonella Vaccine Candidate Inhibits Salmonella Mediated Apoptosis
한국미생물학회 학술대회논문집
2015 .04
-
Transcriptomic Approach for Understanding the Adaptation of Salmonella enterica to Contaminated Produce
Journal of Microbiology and Biotechnology
2020 .01
-
Contemporary Insights into Non-typhoidal Salmonella: Understanding the Pathogenicity, Infection Mechanisms, and Strategies for Prevention and Control
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2024 .12
-
Recent Trends in Salmonella Outbreaks and Emerging Technology for Biocontrol of Salmonella Using Phages in Foods: A Review
Journal of Microbiology and Biotechnology
2017 .01
-
Understanding Comprehensive Transcriptional Response of Salmonella enterica spp. in Contact with Cabbage and Napa Cabbage
Journal of Microbiology and Biotechnology
2018 .01
-
Comparison of Gold Biosensor Combined with a Light Microscope Imaging System with ELISA for Detecting Salmonella in Chicken After Exposure to Simulated Chilling Condition
Journal of Microbiology and Biotechnology
2023 .02
-
Loop-mediated isothermal amplification (LAMP)법을 이용한 Vibrio alginolyticus의 신속 진단법 개발
생명과학회지
2015 .08
-
Quantification of Viable Salmonella enterica by Viability-PCR
한국미생물학회 학술대회논문집
2022 .10
-
LAMP의 개요 및 사용 방법
한국대기환경학회 학술대회논문집
2023 .10
-
Relationship of between Iron and Salmonella inside Macrophage
한국미생물학회 학술대회논문집
2019 .04
-
Complete genome sequence of Salmonella Thompson strain MFDS1004024 isolated from crab-stick
미생물학회지
2018 .06
-
Effect of Indigenous Microbiome in Soil on Salmonella enterica Survival
한국미생물학회 학술대회논문집
2016 .04
-
Uptake of Host Metabolic Succinate Correlates with Salmonella Virulence Induction
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2021 .03
-
Salmonella Single-cell Dynamics in Complex Host Tissues
한국미생물학회 학술대회논문집
2016 .04
-
Non-Invasive Colorimetric Magneto Loop-Mediated Isothermal Amplification (CM-LAMP) Method for Helicobacter pylori Detection
Journal of Microbiology and Biotechnology
2021 .01
-
Role of Hepcidin in Salmonella Infection
한국미생물학회 학술대회논문집
2016 .04
-
Rapid Determination of Salmonella enterica Serotypes Using Pan-genome Based on Real-Time PCR Method
한국미생물학회 학술대회논문집
2019 .04
이 논문의 저자 정보
최근 본 자료
댓글(0)
0