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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제7호
- 발행연도
- 2019.1
- 수록면
- 1,165 - 1,176 (12page)
이용수
초록· 키워드
오류제보하기
Botulinum neurotoxins (BoNTs), produced by Clostridium botulinum, are the most toxic substances known. However, the number of currently approved medical countermeasures for these toxins is very limited. Therefore, studies on therapeutic antitoxins are essential to prepare for toxin-related emergencies. Currently, more than 10,000 Halla horses, a crossbreed between the native Jeju and Thoroughbred horses, are being raised in Jeju Island of Korea. They can be used for equine antitoxin experiments and production of hyperimmune serum against BoNT/A1. Instead of the inactivated BoNT/A1 toxoid, Halla horse was immunized with the receptor-binding domain present in the C-terminus of heavy chain of BoNT/A1 (BoNT/A1-HCR) expressed in Escherichia coli. The anti-BoNT/A1-HCR antibody titer increased rapidly by week 4, and this level was maintained for several weeks after boosting immunization. Notably, 20 μl of the week-24 BoNT/A1-HCR(-immunized) equine serum showed an in vitro neutralizing activity of over 8 international units (IU) of a reference equine antitoxin. Furthermore, 20 μl of equine serum and 100 μg of purified equine F(ab’)2 showed 100% neutralization of 10,000 LD50 in vivo. The results of this study shall contribute towards optimizing antitoxin production for BoNT/A1, which is essential for emergency preparedness and response.
목차
등록된 정보가 없습니다.
참고문헌 (67)
참고문헌 신청
Anderson PD / 2012 / Bioterrorism : toxins as weapons / J. Pharm. Pract 25:121~129
Jernigan JA / 2001 / Bioterrorism-related inhalational anthrax : the first 10 cases reported in the United States / Emerg. Infect. Dis 7:933~944
Shukla HD / 2005 / Clostridium botulinum : a bug with beauty and weapon / Crit. Rev. Microbiol 31:11~18
Tucker JB / 1999 / Historical trends related to bioterrorism : an empirical analysis / Emerg. Infect. Dis 5:498~504
Hedeland M / 2011 / Confirmation of botulism in birds and cattle by the mouse bioassay and Endopep-MS / J. Med. Microbiol 60:1299~1305
Jernigan JA / 2001 / Bioterrorism-related inhalational anthrax : the first 10 cases reported in the United States / Emerg. Infect. Dis 7:933~944
Shukla HD / 2005 / Clostridium botulinum : a bug with beauty and weapon / Crit. Rev. Microbiol 31:11~18
Tucker JB / 1999 / Historical trends related to bioterrorism : an empirical analysis / Emerg. Infect. Dis 5:498~504
Hedeland M / 2011 / Confirmation of botulism in birds and cattle by the mouse bioassay and Endopep-MS / J. Med. Microbiol 60:1299~1305
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