지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 141 - 149 (9page)
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초록· 키워드
오류제보하기
Background: Flow cytometry (FCM) is commonly used to identify many cell populations. We developed a white blood cell (WBC) differential counting system for detecting abnormal cells using FCM incorporating 10 colors and 11 antibodies in a single tube, called “10-color LeukoDiff,” and evaluated its performance. Methods: Ninety-one EDTA-anti-coagulated peripheral blood samples from 76 patients were analyzed using 10-color LeukoDiff. We compared 10 color LeukoDiff results with the results of manual differential count (manual diff). WBCs were classified into 17 cell populations: neutrophils, total lymphocytes, T lymphocytes, B lymphocytes, CD5 and CD19 co-expressing lymphocytes, natural killer cells, total monocytes, 16+ monocytes, eosinophils, immature granulocytes, basophils, myeloblasts, B-blasts, T-blasts, myeloid antigen-positive B-blasts, CD19- plasma cells, and 19+ plasma cells. Results: The correlations between the 10-color LeukoDiff and manual diff results were strong (r>0.9) for mature neutrophils, lymphocytes, eosinophils, immature granulocytes, and blasts and moderate for monocytes and basophils (r=0.86 and 0.74, respectively). There was no discrepancy in blast detection between 10-color LeukoDiff and manual diff results. Furthermore, 10-color LeukoDiff could differentiate the lineage of the blasts and separately count chronic lymphocytic leukemic cells and multiple myeloma cells. Conclusions: The 10-color LeukoDiff provided an accurate and comprehensive WBC differential count. The most important ability of 10-color LeukoDiff is to detect blasts accurately. This system is clinically useful, especially for patients with hematologic diseases, such as acute leukemia, chronic lymphocytic leukemia, and multiple myeloma. Application of this system will improve the development of FCM gating strategy designs.
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등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Meintker L / 2013 / Comparison of automated differential blood cell counts from Abbott Sapphire, Siemens Advia 120, Beckman Coulter DxH 800, and Sysmex. XE-2100 in normal and pathological samples / Am J Clin Pathol 139:641~650
Eilertsen H / 2013 / The usefulness of blast flags on the Sysmex XE-5000 is questionable / Am J Clin Pathol 139:633~640
Barnes PW / 2005 / The International Consensus Group for Hematology review : suggested criteria for action following automated CBC and WBC differential analysis / Lab Hematol 11:83~90
Novis DA / 2006 / Laboratory productivity and the rate of manual peripheral blood smear review : a College of American Pathologists Q-Probes study of 95, 141 complete blood count determinations performed in 263 institutions / Arch Pathol Lab Med 130:596~601
김아현 / 2014 / White blood cell differential counts in severely leukopenic samples: a comparative analysis of different solutions available in modern laboratory hematology / Blood Research 49(2):120~126
Eilertsen H / 2013 / The usefulness of blast flags on the Sysmex XE-5000 is questionable / Am J Clin Pathol 139:633~640
Barnes PW / 2005 / The International Consensus Group for Hematology review : suggested criteria for action following automated CBC and WBC differential analysis / Lab Hematol 11:83~90
Novis DA / 2006 / Laboratory productivity and the rate of manual peripheral blood smear review : a College of American Pathologists Q-Probes study of 95, 141 complete blood count determinations performed in 263 institutions / Arch Pathol Lab Med 130:596~601
김아현 / 2014 / White blood cell differential counts in severely leukopenic samples: a comparative analysis of different solutions available in modern laboratory hematology / Blood Research 49(2):120~126
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