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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.3
- 수록면
- 51 - 56 (6page)
- DOI
- 10.7841/ksbbj.2020.35.1.51
이용수
초록· 키워드
오류제보하기
In this study, the medium composition, carbon source, non-natural amino acid concentration, and induction condition were optimized for the production of recombinant urate oxidase with non-natural amino acids. Recombinant Escherichia coli strain was first cultured in various media including LB, 2 × YT and B, and cell growth and urate oxidase expression were compared. Based on the selected B medium, the concentrations of each component in medium were optimized through response surface methodology. At the optimal medium compositions, urate oxidase with non-natural amino acids was produced with 64.92 U/mg specific protein activity in flask culture. Among various carbon sources, the highest urate oxidase activity was obtained at the glucose concentration of 5 g/L. The optimal inducer concentrations were 1 mM of IPTG. 1 mM of non-natural amino acids was efficient for urate oxidase production. This results will be useful for the production of therapeutic proteins with prolonged activity.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (19)
참고문헌 신청
Kontermann, R. E. / 2011 / Strategies for extended serum half-life of protein therapeutics / Curr. Opin. Biotechnol 22:868~876
Lund, U. / 2003 / Glomerular filtration rate dependence of sieving of albumin and some neutral proteins in rat kidneys / Am. J. Physiol. Renal. Physiol 284:1226~1234
Kontos, S. / 2012 / Drug development : longerlived proteins / Chem. Soc. Rev 41:2686~2695
Elsadek, B. / 2012 / Impact of albumin on drug delivery-new applications on the horizon / J. Control. Release 157:4~28
Kratz, F. / 2012 / Clinical impact of serum proteins on drug delivery / J. Control. Release 161:429~445
Lund, U. / 2003 / Glomerular filtration rate dependence of sieving of albumin and some neutral proteins in rat kidneys / Am. J. Physiol. Renal. Physiol 284:1226~1234
Kontos, S. / 2012 / Drug development : longerlived proteins / Chem. Soc. Rev 41:2686~2695
Elsadek, B. / 2012 / Impact of albumin on drug delivery-new applications on the horizon / J. Control. Release 157:4~28
Kratz, F. / 2012 / Clinical impact of serum proteins on drug delivery / J. Control. Release 161:429~445
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