지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2021.6
- 수록면
- 165 - 170 (6page)
- DOI
- 10.7841/ksbbj.2021.36.2.165
이용수
초록· 키워드
오류제보하기
Sinorhizobium meliloti β-glucosidase (β-D-glucoside glucohydrolase: EC 3.2.1.21) is an exocellulase and cleavages β-(1-4) glycosidic bonds in poly- and oligosaccharides. It has also high activity toward indican, a precursor to indigo and indirubin used as clothing dyes, cosmetics and antimicrobial agents. Typically, this enzyme induced with IPTG under the control of the corresponding promoter was mainly produced as non-functional aggregates in Escherichia coli XL1-Blue and BL21 (DE3). In this study, expression vectors with a mined constitutive promoter (CEM) from metagenome were employed to increase the soluble expression of S. meliloti β-glucosidase. The resulting efficiency of constitutive expression systems was compared with those of the commonly used inducible systems in terms of the specific activity and solubility ratio. Consequently, recombinant β-glucosidases were mainly expressed in soluble form (82-97%) by the constitutive promoter CEM and thus showed the highest specific activity in the recombinant E. coli XL1-Blue harboring pCEMT.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (22)
참고문헌 신청
Pawlak, K. / 2006 / Blue natural organic dyestuffs—from textile dyeing to mural painting. Separation and characterization of coloring matters present in elderberry, logwood and indigo / J Mass Spectrom 41:613~622
Chiavari, G. / 2005 / Identification of indigo dyes in painting layers by pyrolysis methylation and silylation. A case study: “the dinner of emmaus” by G. Preti / Chromatographia 61:403~408
Le Coz, C. / 2011 / Contact dermatitis / Springer :793~817
Kohno, Y. / 2005 / Production of superoxide radical in reductive metabolism of a synthetic food-coloring agent, indigocarmine, and related compounds / Life sciences 77:601~614
Chung, I. M. / 2005 / Traditional dyeing of natural indigo on the sik fabric / Korean J Sericult Sci 47:31~35
Chiavari, G. / 2005 / Identification of indigo dyes in painting layers by pyrolysis methylation and silylation. A case study: “the dinner of emmaus” by G. Preti / Chromatographia 61:403~408
Le Coz, C. / 2011 / Contact dermatitis / Springer :793~817
Kohno, Y. / 2005 / Production of superoxide radical in reductive metabolism of a synthetic food-coloring agent, indigocarmine, and related compounds / Life sciences 77:601~614
Chung, I. M. / 2005 / Traditional dyeing of natural indigo on the sik fabric / Korean J Sericult Sci 47:31~35
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UCI(KEPA) : I410-ECN-0101-2021-470-001849401